Base auxiliary laser desorption-laser inducing fluorescence flying time mass spectrograph
A matrix-assisted laser and laser-induced fluorescence technology, applied in the field of mass spectrometers, to achieve the effect of single-molecule analysis and high detection sensitivity
Inactive Publication Date: 2009-12-02
XIAMEN UNIV
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However, samples without fluorophores must be derivatized prior to analysis
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[0016] Such as figure 1 As shown, the present invention is provided with a cavity 1, a sampling probe rod 2, a front grid group 3, a rear grid group 4, a photomultiplier tube 5, a microchannel plate electron multiplier 6, a laser 7, and a laser focusing lens 8. Gas laser 9 and gas laser focusing lens 10. Sample injection probe rod 2, front grid group 3, rear grid group 4, photomultiplier tube 5 and microchannel plate electron multiplier 6 are sequentially arranged in cavity 1; laser 7, laser focusing lens corresponding to laser 7 8. The gas laser 9 and the gas laser focusing lens 10 corresponding to the gas laser 9 are arranged outside the cavity, and the cavity wall of the cavity 1 is provided with a quartz window 11 for the laser of the laser 7 to enter and for the sample injection probe 2 to enter and exit. The valve 12, the vacuum port 13 for vacuuming and the quartz window 14 for the laser injection of the gas laser 9; the central axis of the cavity 1 and the central axi...
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A matrix-assisted laser desorption-laser-induced fluorescence time-of-flight mass spectrometer relates to a mass spectrometer. Provided is a matrix-assisted laser desorption-laser-induced fluorescence time-of-flight mass spectrometer with high detection sensitivity and strong resolving power, which can realize single-molecule analysis. It is equipped with a cavity, a sample injection probe rod, a front grid group, a rear grid group, a photomultiplier tube, a microchannel plate electron multiplier, a laser, a laser focusing lens, a gas laser and a gas laser focusing lens. The probe rod, the front and rear grid groups, the multiplier tube and the multiplier are arranged in the cavity; the laser and focusing lens, the gas laser and the focusing lens are arranged outside the cavity, and the cavity wall of the cavity is provided with a laser injection window for the probe rod. The inlet and outlet valves and vacuum pumps; the central axis of the cavity is coaxial with the central axis of the probe rod; the front and rear grid groups are composed of grids that are spaced in parallel and perpendicular to the central axis of the cavity; the central axis of the rear grid group is The central axis of the photomultiplier tube is coaxial.
Description
technical field [0001] The invention relates to a mass spectrometer, in particular to a matrix-assisted laser desorption-laser induced fluorescence time-of-flight mass spectrometer. Background technique [0002] Currently, techniques commonly used in the analysis of biomacromolecules include gel electrophoresis, flow cytometry, and mass spectrometry. Gel electrophoresis can separate DNA fragments with a molecular weight of up to several thousand bp, but for DNA fragments with a molecular weight greater than 20,000 bp, pulsed field gel electrophoresis (PFGE) takes a long time (several hours) to complete a detection , the efficiency is low. Moreover, this technique requires a large amount of sample, usually at the μg level, which greatly increases the workload and time of sample processing. In addition, the resolution of gel electrophoresis is poor. For DNA fragments below 1,000 bp, the resolution is about tens of bp, while for DNA fragments above 10,000 bp, the resolution i...
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IPC IPC(8): H01J49/40H01J49/26
Inventor 杭纬何坚颜晓梅张洁
Owner XIAMEN UNIV