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Demanganization functional bacterium

A technology of functional bacteria and Pseudomonas genus, applied in the field of microorganisms, can solve the problems of long recovery period of engineering bacteria, slow filtration speed, long start-up time, etc., and achieve the effects of shortening the establishment time, ensuring quality and speeding up the filtration speed

Inactive Publication Date: 2009-12-23
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that the current bio-oxidative manganese removal engineering bacteria group is established by natural selection, the biochemical ability is weak, the manganese removal effect is poor, and the filtration speed is slow; Changhe Engineering Bacteria has a long recovery period after being destroyed, and provides a manganese-removing functional bacterium

Method used

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  • Demanganization functional bacterium
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specific Embodiment approach 1

[0017] Specific embodiment 1: In this embodiment, the manganese-removing functional bacterium MB32 (Pseudomonas sp.) belongs to the genus Pseudomonas, and is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. The preservation date is October 30, 2007, and the preservation number is It is CGMCC No.2238; the manganese-removing functional bacterium MB32 (Pseudomonas sp.) is a Gram-negative facultative anaerobic bacterium, straight or curved, short rod-shaped, 1-3 μm long, 1-1.5 μm wide, with two cells blunt end (eg figure 1 and figure 2 shown); milky white colonies formed on the culture medium, opaque, with irregular edges, and slightly blue around; manganese-removing functional bacteria MB32 (Pseudomonassp.) has energy storage particles, spores and capsules, N, N, N', N '-tetramethyl-p-phenylenediamine (N, N, N', N'-Tetramethyl-p-Phenylenedia, TMPD) staining was positive.

[0018] Manganese-removing functional bacter...

specific Embodiment approach 2

[0020] Specific embodiment 2: In this embodiment, the manganese-removing functional bacteria MB32 (Pseudomonas sp.) is screened from mud taken from the aeration tank wall of the biological manganese removal filter of Zhangshi biological manganese removal water plant in Shenyang City, Liaoning Province. 1L medium for screening consists of 0.8g peptone, 0.2g yeast extract, 0.1g MnSO 4 .H 2 O, 0.1 g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaNO 3 , 0.1g CaCl 2 , 0.1g (NH 4 ) 2 CO 3 , 0.01g FeSO 4 ·7H 2 O and the remainder of distilled water; then sterilized.

[0021] The present embodiment adopts the combination of plate streaking and plate coating method to divide bacteria, and adopts TMPD method (Vandenabeele J, D de Beer et al. Manganese oxidation by microbial consortia from sand filters. Microb. Ecol. 1992, 24: 91-108. ) for the primary screening of the isolated strains. The principle of using the TMPD method in this embodiment is that N, N, N', N'-tetramethyl-p-phe...

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Abstract

A manganese-removing functional bacterium relates to a microorganism. It solves the problem that the current biological oxidation manganese removal engineering bacteria group is established by natural selection, has weak biochemical ability and poor manganese removal effect; the establishment of water treatment manganese removal biofilter requires long cultivation, domestication, start-up time and engineering bacteria group is destroyed The problem of long recovery period. The manganese-removing functional bacteria MB32 (Pseudomonas sp.) belongs to the genus Pseudomonas and was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date is October 30, 2007, and the preservation number is CGMCCNo.2238; the strain MB32 is Gram-negative facultative anaerobic bacteria, straight or curved, short rod-shaped, 1-3 μm long, 1-1.5 μm wide, blunt at both ends of the cells; milky white colonies formed on the medium, opaque, with irregular edges , slightly blue around; strain MB32 has energy storage granules, spores and capsules, N, N, N', N'-tetramethyl-p-phenylenediamine staining is positive. The manganese-removing functional bacteria MB32 of the present invention has the ability to remove Mn2+ in water, and the removal rate of Mn2+ is higher than 92%.

Description

technical field [0001] The present invention relates to a microorganism. Background technique [0002] Manganese is an abundant metal element in the earth's crust. The concentration of manganese ions in ordinary water is 0.1-1 mg / L, while the concentration of soluble manganese ions in anaerobic groundwater can be as high as 7-9 mg / L. Soluble manganese can form insoluble precipitates (such as MnO 2 or MnOOH). [0003] MnO 2 is a highly insoluble substance (δ-MnO 2 The solubility kso is only 10 -56 ), which is dark black and can cause significant coloration in groundwater intended for drinking; manganese must therefore be removed from drinking water. my country stipulates that the maximum concentration of manganese ions in drinking water is 0.1mg / L, while the European Union has determined the maximum concentration of manganese ions in drinking water as 0.05mg / L. [0004] The removal of soluble Mn(II) from groundwater is usually accompanied by oxidation of manganese ions,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/20
Inventor 张杰赵焱李冬
Owner HARBIN INST OF TECH