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Preparation method of biofertilizer

A bio-fertilizer and probiotic technology, applied in the direction of organic fertilizers, can solve the problems of low nutrient utilization, pollution, improper treatment of culture medium residues, etc.

Inactive Publication Date: 2007-07-18
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nutrient utilization rate of housefly larvae to bran, rice bran and other medium is very low, so the medium residue after cultivating fly maggots still has a high nutritional content, but these medium residues can no longer be used as feed for other animals. Improper handling of these media residues can be a source of contamination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] In the fermentation tank, use liquid medium (LB medium) to cultivate Saccharomyces cerevisiae respectively; in the fermentation tank, add 30L of the above bacterial liquid to 300 kg of bran and 900 liters of water passing through a 15-mesh sieve, mix well, and use Seal the film and ferment for 1 day; spread the fermented bran to form a culture material layer of about 5cm; spread the clean fly eggs on the culture material surface, the inoculation density is 20,000-25,000 eggs / square meter, and the fly eggs hatch They are larvae; cultured at room temperature 26°C for 3.5 days, the fly maggots develop into mature larvae using the nutrition of the compost.

[0014] Spread the compost on the 20-mesh large screen to form a material layer of about 5cm. The upper layer is placed under the fluorescent lamp. Because the fly maggots have negative phototaxis, the fly maggots crawl through the screen and enter the dark bottom space by themselves. This process takes time. About 12h. ...

example 2

[0017] In the fermenter, use liquid medium (LB medium) to cultivate Bacillus licheniformis; in the fermenter, add 30 liters of the above-mentioned bacterial liquid to 300 kilograms of bran and 900 liters of water through a 15-mesh sieve respectively, after fully mixing, Use a film to seal and ferment for 1 day; spread the fermented bran to form a layer of compost of about 5 cm; spread clean fly eggs on the surface of the compost at an inoculation density of 20,000 to 25,000 eggs / square meter. Hatch into larvae; culture at room temperature 26°C for 3.5 days, and the fly maggots develop into mature larvae using the nutrition of the compost.

[0018] Spread the compost on the 20-mesh large screen to form a material layer of about 5cm. The upper layer is placed under the fluorescent lamp. Because the fly maggots have negative phototaxis, the fly maggots crawl through the screen and enter the dark bottom space by themselves. This process takes time. About 12h. The harvested fly ma...

example 3

[0021] In the fermentation tank, use liquid medium (LB medium) to cultivate Enterobacter cloacae; in the fermentation tank, add 30L of the above bacterial solution to 300 kg of bran and 900 liters of water that have passed through a 15-mesh screen, mix well, and use Seal the film and ferment for 1 day; spread the fermented bran to form a culture material layer of about 5cm; spread the clean fly eggs on the culture material surface, the inoculation density is 20,000-25,000 eggs / square meter, and the fly eggs hatch They are larvae; cultured at room temperature 26°C for 3.5 days, the fly maggots develop into mature larvae using the nutrition of the compost.

[0022] Spread the compost on the 20-mesh large screen to form a material layer of about 5cm. The upper layer is placed under the fluorescent lamp. Because the fly maggots have negative phototaxis, the fly maggots crawl through the screen and enter the dark bottom space by themselves. This process takes time. About 12h. The ...

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PUM

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Abstract

This invention provides a method of using housefly larvae and life-profit-germ to handle bran for preparation of biological fertilizer. It includes the following process: (1) adding life-profit-germ into bran for culture; (2) put the bran after cultivation in housefly larvae breed plate, add housefly eggs; (3) In the 20-30 deg C for 3 to 5 days, isolate housefly larvae; (4) seal the cultivation material after isolating housefly larvae for fermentation to kill the remaining frogeye maggot, then obtain microbial fertilizer; (5) drying and packaging.

Description

technical field [0001] The invention relates to a method for treating bran with housefly larvae and probiotics to obtain biological fertilizer. Background technique [0002] The housefly (Musca domestica) belongs to Insecta, Diptera, Cylindrica, and Muscaidae. It has the advantages of strong fecundity, short life cycle, abundant feed sources, and easy intensive production. The first protein resource. [0003] During the cultivation of housefly larvae (maggots), agricultural by-products such as bran are generally used as culture medium. The nutrient utilization rate of housefly larvae to bran, rice bran and other medium is very low, so the medium residue after cultivating fly maggots still has a high nutritional content, but these medium residues can no longer be used as feed for other animals. Improper handling of these media residues can be a source of contamination. If these medium residues can be used as organic fertilizer, it will not only solve the problem of how to ...

Claims

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Application Information

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IPC IPC(8): C05F11/00C05F11/08
Inventor 韩日畴陈镜华曹莉刘小芬
Owner GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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