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Kit for screening carrier of vertebra malformation syndrome of Holland milch cow

A kit and syndrome technology, applied in the field of kits for screening Holstein dairy cattle vertebral deformity syndrome carriers, can solve the problems of cow elimination, economic loss, and the inability to accurately locate individual cows, etc. The effect of reliable test results

Inactive Publication Date: 2007-07-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to CVM, more than half of the cows' abortions occur in 100-260 days after conception, which is the peak milk production period of cows. Abortion makes it difficult for cows to reach the peak milk production, resulting in unplanned elimination of cows. resulting in serious economic losses
However, only based on pedigree analysis, it is impossible to accurately locate whether an individual dairy cow is a CVM carrier, but only an inference at the group level

Method used

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  • Kit for screening carrier of vertebra malformation syndrome of Holland milch cow

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1. Using the kit for screening carriers of Holstein spondylotic syndrome to screen some Chinese Holstein spondylotic syndrome carriers

[0026] 1. Screening kit for carriers of Holstein dairy cow vertebral deformity syndrome

[0027] The kit for screening Holstein dairy cows with vertebral deformity syndrome carriers includes PCR reaction reagents, polyacrylamide gel electrophoresis reagents and silver staining reagents. Their specific composition is as follows:

[0028] 1. PCR reagents:

[0029] Taq DNA polymerase (2.5U / uL), 10×PCR buffer (containing Mg 2+ ), dNTPs (4×2.5mM) were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd., and their catalog numbers were ET101-02 and CD111-02 respectively; the primer concentration was 25umol / L. Wherein, the sequence of the forward primer is: AGC TCT CCT CTG TAA TCC (sequence 1), and the sequence of the reverse primer is: TCT CAA AGT AAA CCC CAG (sequence 2).

[0030] 50×TAE: Tris base 242g, glacial ace...

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Abstract

This invention discloses a test kit for screening complex vertebral malformation carriers of Holstein cows. The test kit comprises PCR-SSCP analysis reagent, which comprises PCR primers for amplifying 5' side 41-200bp and 3' side 45-328bp deoxyribonucleotides of 559th deoxyribonucleotide of SLC35A3 gene exon 4. The test kit has such advantages as convenient usage, rapid detection, high sensitivity, reliable results, high stability, and high accuracy. When it is used to screen complex vertebral malformation carriers of Holstein cows, no special device is required, which can meet the needs of laboratory detection.

Description

technical field [0001] The invention relates to a kit for screening carriers of Holstein dairy cow vertebral deformity syndrome. Background technique [0002] Holstein cows are an important breed of livestock that provide human milk. After a high degree of selective breeding and good feeding management, they produce a large amount of fresh milk to meet our food needs. Due to the outstanding results of genetic breeding and artificial insemination technology, potential high-quality bulls are used to produce a large amount of frozen semen, which are used for breeding many cows, and their excellent genetic performance can be quickly spread to all parts of the world. If you trace the pedigree of Holstein cows all over the world, most of their relatives come densely from a few specific bull families. However, the application of artificial insemination technology has made the spread of genetic defects faster and wider. [0003] Genetic defects refer to defects that can be passed ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张沅孙东晓初芹
Owner CHINA AGRI UNIV