Method for detecting small and dense low density lipoprotein with microflow hole chip electrophoretic separation

A low-density lipoprotein, microfluidic chip technology, applied in the direction of separation methods, dispersed particle separation, chemical instruments and methods, etc., can solve the problems of complex operation, time-consuming technical requirements, no separation of sLDL, etc., and achieve the effect of simple operation

Inactive Publication Date: 2011-07-27
AFFILIATED HOSPITAL OF NANTONG UNIV
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Problems solved by technology

Ultracentrifugation is currently the reference method for separating sLDL, but it is not easy to carry out in ordinary laboratories due to the need for expensive equipment, complicated operation, time-consuming and high technical requirements
So far there is no simple, easy, fast and accurate method for separating sLDL

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  • Method for detecting small and dense low density lipoprotein with microflow hole chip electrophoretic separation
  • Method for detecting small and dense low density lipoprotein with microflow hole chip electrophoretic separation
  • Method for detecting small and dense low density lipoprotein with microflow hole chip electrophoretic separation

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Embodiment Construction

[0015] A method for microfluidic chip electrophoresis separation and detection of small and dense low-density lipoproteins, comprising the following steps:

[0016] (1) Specimen pretreatment: Dissolve 5 μl serum sample in 15 μl deionized water, then prestain with 0.5 mg / ml NBD C6-ceramide 10 μl for 1 minute, then add pH9.8 sample Tricine buffer, sample Tricine buffer consists of 40 mol / ml The Tricine of 1, the diatrizoate meglumine of 40mol / l, the SDS composition of 0.2mmol / l, obtain the sample to be tested;

[0017] (2) A cross-shaped microfluidic chip is used; the chip is made of quartz glass, which is made by photolithography, wet etching, and low-temperature bonding. The depth is about 30 μm, the length from the chip injection pool to the intersection point is 28 mm, the effective separation length is 45 mm, and the diameter of the liquid storage pool is 2 mm. Dip the sample pool 1, sample waste pool 2, buffer pool 3, buffer waste pool 4 and channels of the microfluidic c...

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Abstract

This invention discloses a method for separating and detecting small and intense fat protein through electrophoresis of a microfluidic chip, which comprises: a sample pre-processing the microfluidic chip, performing electrophoresis by an electrophoresis apparatus, separating the small and intense fat protein from the sample of the hyperlipoproteinemia patient. The invention can simply, easily, rapidly and accurately separate and detect the small and intense fat protein, only need six minutes from the sample prestained to the result analysis. The chip can be used for thousands of times, and the operation is simple.

Description

Technical field: [0001] The invention relates to a method for detecting small and dense low-density lipoprotein. Background technique: [0002] Lipoproteins are nanometer-sized spherical particles, cholesteryl esters and triglycerides form a hydrophobic inner shell, and apolipoproteins, phospholipids, and free cholesterol form a hydrophilic outer shell. Plasma lipoproteins were divided into chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) by ultracentrifugation. Low high-density lipoprotein cholesterol and high low-density lipoprotein cholesterol have been recognized as traditional risk factors for coronary heart disease. In recent years, studies have found that low-density lipoprotein has obvious heterogeneity, and small dense low density lipoprotein (sLDL) has a stronger arteriogenesis effect than large buoyant low density lipoprotein (LDL1). The effect of atherosclerosis, sLDL is more susceptible to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68B01D57/02
Inventor 王惠民汪骅丛辉孙承龙张志泉褚少朋
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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