Application of ferricitras in preparation of medicine to prevent and treat angiosteosis
A technology for vascular calcification and ferric citrate, which is used in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc.
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Embodiment 1
[0138] Embodiment 1: the preparation of ferric citrate
[0139] Referring to the preparation method recorded in the "Food Additive Handbook" Chemical Industry Press, ferric citrate was prepared by reacting ferric hydroxide with citric acid.
[0140] Weigh 106.9g of micronized ferric hydroxide and 220.7g of citric acid monohydrate, put them in a stirred flask, add 1000ml of water, heat to 60°C in a water bath, and continue to stir for 72 hours. To judge the end point of the reaction, absorb 1ml Add 10ml of water to the supernatant, and detect with spectrophotometry, the solution is basically clear and reaches the end of the reaction.
[0141] The reaction solution was concentrated below 60°C until the relative density of the solution was 1.26 to obtain a brown viscous concentrated solution.
[0142] The concentrated solution was dried under reduced pressure below 60°C to obtain 307 g of crude ferric citrate.
[0143] Put the above-mentioned ferric citrate in a beaker, add 300...
Embodiment 2
[0144] Embodiment 2: Acute toxicity test of ferric citrate mice
[0145] On the basis of the pre-test, 70 Kunming mice (19-21 g, half male and half male) were randomly divided into 7 groups after fasting for 15 hours, with 10 mice in each group. With 4% starch paste, the iron citrate prepared in Example 1 was formulated into uniform suspensions of 7 concentrations, and 7 dosage groups were set up, and the dosages from high to low were: 10.00, 8.00, 6.40, 5.12, 4.10, 3.28 and 2.62 g / kg. The mice were given intragastric administration at 20ml / kg respectively, and the animal's activities, food intake, skin, mucous membranes, feces and death were observed and recorded immediately after the administration, and observed at least once a day within two weeks after the administration. Autopsies were performed on the dead animals, and the changes of various tissues and organs were observed. The median lethal dose was calculated by Bliss method. The results are shown in Table 8 below;...
Embodiment 3
[0159] Embodiment 3: the research of ferric citrate treatment arterial calcification
[0160] The ferric citrate prepared in Example 1 was made into a suspension with 4% starch solution, and administered by intragastric administration according to different dosages.
[0161] Experimental method According to the method of Niederhoffer, an animal model of vascular calcification in rats was prepared.
[0162] 54 male SD rats, weighing 150-180g, were randomly divided into 5 groups: (1) Vascular calcification group (VDN) (n=12): intramuscular injection of vitamin D to the rats at 9:00 on the first day of the experiment 3 (3×10 5 U / kg) and nicotine dissolved in soybean oil (25mg / kg, 5ml / kg) were administered orally, and nicotine was repeatedly administered once at 18:00. Stomach, for 4 consecutive weeks; (2) Blank control group (control, n=10): given intramuscular injection of the same amount of soybean oil, or intragastric administration of simple soybean oil instead of vitamin D...
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