Process of extracting soluble polysaccharide from fragile blueberry leaf

A black rice leaf, soluble technology, applied in the field of extraction of soluble polysaccharides, can solve the problems that the extraction process has not been reported, and achieve the effect of simple process, high extraction rate and improved extraction rate

Inactive Publication Date: 2007-08-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reports on the research on the nutritional components of the black rice tree or plants of the same ge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Embodiment 1, a kind of method that extracts soluble polysaccharide from black rice leaf, carries out following steps successively:

[0016] 1), drying and crushing: after cleaning and drying 100g of fresh black rice leaves, put them in an oven at 50°C for 24 hours and then crush them with a plant grinder. The speed of the plant grinder is 1400r / min, and the crushing time is 25min. The powder can be passed through a 120-mesh sieve; 13.9 g of black rice leaf powder is obtained.

[0017] 2), leaching: after mixing the above-mentioned black rice leaf powder and distilled water in a weight ratio of 1:5, hot leaching in a hot water bath at 30°C for 1 hour, cooling to room temperature, and filtering to obtain the filtrate;

[0018] 3), centrifugation: centrifuge the above filtrate at 3000r / min for 20min to obtain liquid phase I and precipitate I;

[0019] 4) Washing: Mix and stir the liquid phase I and 80% alcohol at a weight ratio of 1:5, centrifuge at 3000r / min for 20min, ...

Example Embodiment

[0023] Embodiment 2, a kind of method for extracting soluble polysaccharide from black rice leaf, carries out following steps successively:

[0024] 1) Drying and crushing: 100g of fresh black rice leaves are cleaned and dried, put in an oven at 60°C for 36 hours, and then crushed with a plant grinder. The speed of the plant grinder is 1400r / min, and the crushing time is 35min. The crushed powder It can be passed through a 120-mesh sieve to obtain 14.1 g of black rice leaf powder.

[0025] 2), leaching: after mixing the above-mentioned black rice leaf powder and distilled water in a weight ratio of 1:10, hot leaching in a hot water bath at 100°C for 3 hours, cooling to room temperature, and filtering to obtain the filtrate;

[0026] 3), centrifugation: centrifuge the above filtrate at 3100r / min for 30min to obtain liquid phase I and precipitate I;

[0027] 4) Washing: Mix and stir the liquid phase I and 80% alcohol at a weight ratio of 1:10, centrifuge at 3100r / min for 20min,...

Example Embodiment

[0031] Embodiment 3, a kind of method for extracting soluble polysaccharide from black rice leaf, carries out following steps successively:

[0032] 1), drying and crushing: 100g of fresh black rice leaves are cleaned and dried, put in an oven at 55°C for 30 hours, and then crushed with a plant grinder. The speed of the plant grinder is 1400r / min, and the crushing time is 30min. The crushed powder It can be passed through a 120-mesh sieve to obtain 14.0 g of black rice leaf powder.

[0033] 2), leaching: after mixing the above-mentioned black rice leaf powder and distilled water in a weight ratio of 1:20, hot leaching in a hot water bath at 65°C for 5 hours, cooling to room temperature, and filtering; the filtrate was obtained;

[0034] 3), centrifugation: centrifuge the above filtrate at 2900r / min for 25min to obtain liquid phase I and precipitate I;

[0035] 4) Washing: Mix and stir the liquid phase I and alcohol with a concentration of 80% in a weight ratio of 1:8, centrif...

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Abstract

The process of extracting soluble polysaccharide from fragile blueberry leaf includes the following steps: 1. drying fragile blueberry leaf and crushing in a crusher; 2. leaching fragile blueberry leaf powder in hot distilled water, cooling and filtering to obtain filtrate; 3. centrifuging the filtrate to obtain liquid phase and precipitate; 4. washing the liquid phase through mixing with 80 % concentration alcohol solution and centrifuging to obtain fragile blueberry polysaccharide; 5. washing the liquid phase through mixing with 80 % concentration alcohol solution and centrifuging to obtain fragile blueberry polysaccharide repeatedly for 1-3 times; and 6. vacuum drying the collected fragile blueberry polysaccharide. The present invention has simple technological process and high extracting rate.

Description

technical field [0001] The invention relates to a method for extracting soluble polysaccharides, in particular to a method for extracting soluble polysaccharides from leaves of rhododendronaceae. Background technique [0002] Black rice tree is a plant of the genus Vaccinium in the Rhododendron family. It is widely distributed in my country and has a large reserve. It is mainly produced in East China, Central China, and South China. It is often distributed in hilly areas or hillside forests or bushes at an altitude of 400-1400m. Among them, it is an indicator plant for acidic soil (red soil). It is reported that it has the effects of enhancing memory, anti-fatigue and cold resistance, and has improvement and preventive effects on myopia, senile cataract, and diabetes. Many studies have shown that plant polysaccharides have biological activities such as anti-tumor, hypoglycemic, hypolipidemic, and anticoagulant, while the anti-tumor, hypoglycemic, and hypolipidemic biological...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 朱加进施佳慧程俊文
Owner ZHEJIANG UNIV
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