Microorganism metabolism control of actinoplanes species

A technology of microbial metabolism and swimming actinomycetes, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of shortened product synthesis stages, high nutrient concentration, and reduced output of metabolites, and achieve easy industrialization The effect of high production and fermentation level and low production cost

A technology of microbial metabolism and swimming actinomycetes, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of shortened product synthesis stages, high nutrient concentration, and reduced output of metabolites, and achieve easy industrialization The effect of high production and fermentation level and low production cost

CN101041806AActive Publication Date: 2007-09-26HUADONG MEDICINE +2
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Periodic feeding: connect the feeding tank and the fermentation tank through pipelines. After the feeding tank is sterilized, sterilize the feeding pipeline. After completion, press 4 tons of material into the fermentation tank through sterile air to complete the feeding. The whole fermentation process then needs 4 times of this feeding process, and the total feeding amount is 16 tons. Put it in the tank and measure the fermentation unit: 3430μg / ml.

[0019] Continuous automatic variable speed feeding: install a distributed control system on the computer, connect the feeding tank and the fermentation tank through pipelines, and install an automatic diaphragm valve in the middle. After the feeding tank is sterilized, start the automatic feeding device to sterilize the feeding pipeline. After the completion, use sterile air to hydraulically pass the material, and pass the automatic diaphragm valve (the computer controls the opening and closing time of the valve according ...

Embodiment 2

[0023] A distributed control system is installed on the computer, the feed tank and the fermenter are connected through pipelines, and an automatic diaphragm valve is installed in the middle. After the feeding tank is sterilized, start the automatic feeding device to sterilize the feeding pipeline. After the completion, use sterile air to hydraulically pass the material, and pass the automatic diaphragm valve (the computer controls the opening and closing time of the valve according to the set feeding amount) ) into the fermenter.

[0024] Continuous automatic constant-speed feeding: From about 40 hours in the early stage of fermentation to about 144 hours in the middle and late stages of fermentation, 2.5L of feed liquid is added per minute, and the total amount of feeding is 15 tons. Put the tank, and measure the fermentation unit 4634μg / ml.

[0025] Continuous automatic variable speed feeding: Feed feeding starts at 44 hours after fermentation, and feeds 3.5L of feed liqui...

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Abstract

The invention discloses a preparing method of high unit acar wave sugar through regulating and controlling microorganism metabolism of flowing actinomycete Actinoplanes species, which comprises the following steps: adopting computer automatic speed changing supplementary material method; supplementing the material from 40-48 h of microbe ferment; supplementing at 3-4 L feed liquid per min; persisting to 84-92 h; adjusting the quantity of supplementary material to 1-2 L feed liquid per minute. This sugar possesses high ferment level and low cost, which does not need to modify original device.

Description

technical field [0001] The invention relates to the field of biochemical industry, and more specifically relates to a method for preparing high-unit acarbose by regulating microbial metabolism. Background technique [0002] Acarbose, chemical name: O-4,6-dideoxy-4-[(1S,4R,5S,6S)4,5,6-trihydroxy-3-(hydroxymethyl)-2 -Cyclohexenyl-1-amino]-a-D-glucopyranosyl-(1→4)-O-a-D-glucopyranosyl-(1→4)-D-glucopyranose, produced by Actinomycetes The metabolites produced by Actinoplanes species are mainly used in the treatment of type II diabetes. [0003] In the industry, acarbose is prepared by regulating the microbial metabolism of Actinoplanes species, that is, by fermentation. The feeding method generally adopted in the microbial fermentation method is periodic feeding, feeding once every other day, and a fermentation cycle takes about 8 days, that is, feeding 3-4 times in a fermentation cycle. This feeding method has great disadvantages. Because it is a one-time supplement of carbon...

Claims

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Application Information

Patent Timeline
26 Sep 2007
Publication
CN101041806A
IPC
C12N1/00; C12P19/26; C12R1/045
Inventors
周鲁瑾; 章飞龙