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Tumor displace mimetism organization chip

A tumor metastasis and tissue chip technology, which is applied in the preparation of material inspection products, test samples, biological tests, etc., can solve the problems of staying, no comparative research on the dynamic changes of cell-related gene expression, etc.

Inactive Publication Date: 2007-09-26
孙爱静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing studies on tumor metastasis remain at the level of comparing the similarities and differences of tumor cells between the metastases and the primary tumors, without detailed studies on the biological state of the cells and the dynamic changes of related gene expressions at each stage of the tumor metastasis process. compare research

Method used

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  • Tumor displace mimetism organization chip
  • Tumor displace mimetism organization chip
  • Tumor displace mimetism organization chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Mimic tissue chip for breast ductal carcinoma lymphatic metastasis

[0030] (1) Collection of donor paraffin tissue specimens:

[0031] According to the WHO (2003) new histological classification of breast tumors, cases of minimally invasive ductal carcinoma and invasive ductal carcinoma (including positive and negative lymph node metastasis) were selected, and surgical resection specimens were collected in recent years.

[0032] The basis for selecting microinvasive carcinoma and invasive ductal carcinoma (including positive and negative lymph node metastasis): ductal carcinoma of the breast occurs in the duct of the breast, and is confined to the duct in the early stage to form ductal carcinoma in situ. As the disease progresses, cells with invasive ability proliferate And break through the basement membrane to form micro-invasive carcinoma, and then, the infiltrating cancer cells infiltrate in the loose and edematous interstitium under the external interac...

Embodiment 2

[0047] Example 2: Colorectal Adenocarcinoma Blood Tract Metastasis Tissue Chip

[0048] (1) Collection of donor paraffin tissue specimens:

[0049] According to WHO (2000) colorectal tumor histology and TNM classification criteria, collected in recent years Tis (carcinoma in situ: intraepithelial or infiltrating lamina propria), T1 (tumor infiltrating submucosa), T2 (tumor infiltrating muscle layer), T3 ( Surgical resection of adenocarcinoma cases (including positive and negative liver metastases) to select paracancerous tissues, cancer tissues with different depths of invasion (invasive cancer and intravascular tumor thrombus), and liver metastases wax block specimen.

[0050] (2) Perform immunohistochemical staining on the above-mentioned donor paraffin tissue specimens:

[0051] T1, T2, and T3 specimens were serially sectioned, and conventional HE staining and lymphatic staining (such as vascular endothelial growth factor receptor-3, vascular endothelial growth factor rec...

Embodiment 3

[0062] Example 3: Gastric cancer tumor implantation and metastasis tissue chip

[0063] (1) Collection of donor paraffin tissue specimens:

[0064] According to the WHO (2000) gastric tumor histology and TNM classification criteria, Tis (carcinoma in situ: confined to the epithelial layer), T1 (tumor infiltrating the lamina propria or submucosa), T2 (tumor infiltrating the muscular layer), and T3 in recent years were collected. (Tumor infiltrating the subserosa without invading adjacent structures) and T4 adenocarcinoma with implantation and metastasis were surgically resected to select paracancerous tissues, tumor tissues infiltrating at different depths, and implanted and metastatic cancer tissues.

[0065] (2) Perform immunohistochemical staining on the above-mentioned donor paraffin tissue specimens:

[0066] T1, T2, T3, and T4 specimens were serially sectioned, and conventional HE staining and lymphatic staining (such as vascular endothelial growth factor receptor-3, vas...

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Abstract

This invention discloses one tumor transfer mimic organism chip, which is processed by the following steps: selecting the supply wax organism specimen through each phase in transfer process and through normal HE dyeing assistant immune set; positioning tumor transfer process each point for label; collecting labeled organism to form chip matrix slice; then processing the silicon slice through process. This invention can test tumor transfer process relative gene expression and its status and filter prediction factors and after factors to guide tumor personal medical and target medicine.

Description

technical field [0001] The invention relates to a tumor metastasis mimetic tissue chip in the field of biological research, which is used for large-scale detection of the expression and change dynamics of related genes in the progress of tumor metastasis, screening of prognostic and predictive factors, and guidance of individualized treatment and targeted treatment. Background technique [0002] Tissue chip (tissue chip), also known as tissue microarray (tissue microarray, TMA), is an emerging biological research technology, first established and reported by Kononen et al. Microscopic tissue sections arranged on a glass slide. A tissue chip wax block can be made into 100-200 serial sections, so that hundreds of biomolecular markers (such as antigens, DNA and RNA) can be analyzed and detected with the same set of tissue chips, such as a patent No. 01128783.7 A tissue microarray biochip that can simultaneously compare and analyze the similarities, differences and changes of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/48
Inventor 孙爱静苏晶石田景琦
Owner 孙爱静
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