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Blood pressure depressed peptide from royal jelly

A technology of royal jelly and protein decomposition products, applied in the fields of food and oral preparations, to achieve the effect of effective prevention or treatment of hypertension, less side effects and strong ACE inhibitory effect

Inactive Publication Date: 2007-10-03
YAMADA BEE COMPANY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these peptides still have room for improvement in the direction of industrialization from the viewpoint of the balance between the amount obtained by hydrolyzing royal jelly materials and the strength of ACE inhibitory activity, quick-acting properties, side effects, duration of action, shape of ingestion, and storage stability.

Method used

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  • Blood pressure depressed peptide from royal jelly

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0316] Using 1 g of ethanol-denatured protein precipitated by adding ethanol to royal jelly, 250 mg of porcine trypsin was added and treated at pH 8.0 for 24 hours. After the reaction, heat at 100° C. for 10 minutes to inactivate the enzyme.

[0317] The reaction solution was adjusted to pH 4.5, applied to a synthetic adsorbent (Sepabis SP-70 (manufactured by Mitsubishi Chemical)), and washed with water to remove sodium chloride, acidic or alkaline free amino acids, sugars, etc. , and then eluted with ethanol to obtain the desalted protein hydrolyzate of the present invention.

[0318] The protein breakdown product after desalination does not substantially contain basic free amino acids, acidic free amino acids, alcoholic free amino acids, free Ala and free Gly, and the residual rate of Lys calculated according to Mathematical Formula 1 is about 45%, calculated according to Mathematical Formula 2 The content ratio (molar basis) of Leu was about 2.3 times. In addition, the su...

Embodiment 2

[0327] Embodiment 2: Structural analysis of the relevant components of royal jelly (RJ) protein hydrolyzate

[0328] 100 g of ethanol-denatured protein of royal jelly was taken, and enzymatically digested with porcine trypsin (pig-derived trypsin manufactured by Novozyme) at pH 8.0 and 37° C. for 24 hours. The reaction solution was heated in a boiling water bath for 30 minutes to inactivate the enzyme, then adjusted to pH 4.5 with hydrochloric acid, centrifuged (10000 rpm, 20 minutes), and the obtained supernatant was filtered with a membrane filter (0.8 μm), 1160 mL of RJ protein hydrolyzate was obtained.

[0329] The obtained RJ protein hydrolyzate was eluted with water, 20%, 40%, 60%, 80% methanol, and methanol (100%) according to the above-mentioned flow chart 1 using Diayion HP20 to obtain Fraction 1 (Fr1) to Point 7 (Fr7).

[0330] Then, a detailed description of the isolation and identification of the 4 related components (peptides) identified from fractions 3-6 is as...

Embodiment 3

[0368] Example 3: Clinical trials of proteolytic products

[0369] In the same manner as in Example 1, a porcine trypsin hydrolyzate of RJ ethanol-denatured protein (containing 4 novel peptides of the present invention) was obtained, which was adsorbed on Diayion HP20 according to the above flow chart, and then washed to remove fraction 1 and 2, and then eluted with 80% ethanol to prepare the protein breakdown product of the present invention containing fractions 3-6, which were provided for the following experiments.

[0370] In order to study the antihypertensive effect and safety of long-term intake of tablets containing the obtained royal jelly protein hydrolyzate (RJPH), a group of people with high blood pressure (no treatment for normal high blood pressure patients and mild hypertensive patients) was carried out. A placebo-controlled double-blind trial. In order to make no difference in gender, age, systolic blood pressure, diastolic blood pressure, pulse rate, body wei...

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Abstract

The invention relates to a protein decomposition product, obtained by processing royal jelly materials by trypsinase, having an inhibiting effect to angiotensin converting enzyme, containing at least one of the following 4 peptides: Thr-Ser-Asn-Thr-Phe; Tyr-Ser-Pro-Val-Ala-Ser-Thr; Thr-Asn-Asn-Leu-Tyr; Val-Pro-Ile-Phe-Asp-Arg. The protein decomposition product in the invention has an inhibiting effect to angiotensin converting enzyme and is effective to the prevention and cure of hypertension.

Description

technical field [0001] The present invention relates to a royal jelly-derived protein breakdown product, a method for producing the same, and foods and oral preparations containing the protein breakdown product. The proteolytic product of the present invention has an angiotensin-converting enzyme inhibitory effect and is effective for the prevention or treatment of hypertension. Background technique [0002] Hypertension is a disease that has no subjective symptoms. If left untreated, it will lead to serious diseases such as heart disease and cerebrovascular disease. The treatment and prevention of hypertension is a major issue. It is known that angiotensin-converting enzyme (hereinafter referred to as ACE) is closely related to hypertension, and hypertension can be prevented or treated by inhibiting ACE. [0003] On the other hand, drugs for the prevention or treatment of high blood pressure need to be ingested for a long period of time, so having extremely low side effect...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P9/12A23L1/305
Inventor 柳田正
Owner YAMADA BEE COMPANY INC
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