Peptide for treating obesity
The technology of a compound, x1-x2-x3-arg-x4-x5, is applied in the field of application of the compound in the preparation of medicines, and can solve problems such as obesity
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Embodiment 1
[0260] 2-[2-(Hexadecanoylamino)ethoxy]ethoxyacetyl-His-Ser-Nle-c[Glu-Asn-D-Phe-Arg-Trp-Lys]-NH 2
[0261]
[0262] Step A of Example 1: Protected Peptide Resin Fmoc-Nle-c[Glu-Asn(Trt)-D-Phe-Arg(Pbf)-Trp-Lys]-NH-Rink Linker-Polystyrene
[0263] Fmoc-Rink resin (4-(2′,4′-dimethoxyphenyl-Fmoc-aminomethyl)-phenoxy polystyrene resin, Bachem D-2080, Lot 514460; 0.47mmol / g) Charge into two 60 ml Teflon reactors with glaze (each reactor: 3.55 g, 1.67 mmol). The resin in each reactor was washed with DCM (30ml).
[0264] Branching of Fmoc: The resin was shaken with 20% piperidine in NMP (25 ml) for 20 min, then washed with NMP / DCM 1:1 (5 x 30 ml).
[0265] Acylation with Fmoc-Lys(Mtt)-OH: In a separate glass vial, Fmoc-amino acid (10.0 mmol) was mixed with NMP (12.5 ml), DCM (22.5 ml) and 1M 1-hydroxybenzotriazole ( HOBt) in NMP (10.0 ml, 10.0 mmol) were mixed. To the resulting clear solution was added DIC (1.56ml, 10.0mmol) rapidly and the solution was shaken immediately. The ...
Embodiment 2
[0286] 2-[2-(Hexadecanoylamino)ethoxy]ethoxyacetyl-Gln-Ser-Nle-c[Glu-Asn-D-Phe-Arg-Trp-Lys]-NH 2
[0287]
[0288] LCMS (system 1): Rt = 4.49 min; ((m+2) / 2) = 787.
Embodiment 3
[0290] 2-[2-(2-{2-[2-(tetradecanoylamino)ethoxy]ethoxy}acetylamino)ethoxy]ethoxyacetyl-Nle-c[Glu-Asn -D-Phe-Arg-Trp-Lys]-NH 2
[0291]
[0292] LCMS (system 1): Rt = 4.30 min; ((m+2) / 2) = 738.
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