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Simulated four-area moving bed chromatographic process for separating and purifying aloe flavone

A technology of moving bed chromatography and simulated moving bed, applied in the field of separation and purification of aloe flavonoids by four-zone simulated moving bed chromatography, to achieve the effect of improving the production environment and increasing the level of automation

Active Publication Date: 2007-11-07
江苏汉邦科技股份有限公司
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  • Application Information

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Problems solved by technology

However, there is still no public report on the method of using simulated moving bed chromatography to separate and purify aloe flavonoids

Method used

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Examples

Experimental program
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Effect test

example 1

[0013] Example 1: Separation and purification of aloe flavonoids on the simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0014] a, get 0.5kg dry aloe in 5kg hot water ultrasonic leaching, filter to obtain filtrate;

[0015] b. Add equal volume of ethyl acetate to the filtrate, carry out liquid-liquid extraction at room temperature for 3 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of aloe flavonoids;

[0016] c. The crude extract of aloe flavonoids is made into a sample liquid and entered into a simulated moving bed for separation and purification; the crude extract of aloe flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic filter membrane to obtain a feed liquid of a simulated moving bed with a concentration of 100 mg / ml; elute The liquid is a mixture of methanol and water, and the volume ratio of methanol to water is 45:55;

[0017] d. Vacuum-c...

example 2

[0021] Example 2: Separation and purification of aloe flavonoids on the simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0022] a, get 0.8kg dry aloe in 8kg hot water ultrasonic leaching, filter to obtain filtrate;

[0023] b. Add equal volume of ethyl acetate to the filtrate, carry out liquid-liquid extraction at normal temperature for 4 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of aloe flavonoids;

[0024] c. The crude extract of aloe flavonoids is made into a sample liquid and entered into a simulated moving bed for separation and purification; the crude extract of aloe flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic filter membrane to obtain a feed liquid of a simulated moving bed with a concentration of 200 mg / ml; elute The liquid is a mixture of methanol and water, and the volume ratio of methanol to water is 45:55;

[0025] d. Vacuum...

example 3

[0029] Example 3: Separation and purification of aloe flavonoids on the simulated moving bed of Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0030] a, get 1kg dry aloe and 10kg hot water ultrasonic extraction, filter to obtain the filtrate;

[0031] b, add equal volume of ethyl acetate to the filtrate, carry out liquid-liquid extraction at normal temperature for 5 hours, filter, and evaporate the filtrate to dryness under reduced pressure to obtain the crude extract of aloe flavonoids;

[0032] c. The crude extract of aloe flavonoids is made into a sample liquid and entered into a simulated moving bed for separation and purification; the crude extract of aloe flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic filter membrane to obtain a feed liquid of a simulated moving bed with a concentration of 300 mg / ml; elute The liquid is a mixture of methanol and water, and the volume ratio of methanol to water is 45:55;

[0033] d. ...

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Abstract

The present invention discloses simulated four-area moving bed chromatographic process for separating and purifying aloe flavone. The process includes the following steps: ultrasonically leaching aloe in hot water and filtering to obtain filtrate; adding ethyl acetate into the filtrate for extracting at normal temperature, filtering and vacuum distilling to obtain coarse aloe flavone extract; separating and purifying the coarse aloe flavone extract dissolved in methanol in a simulated moving bed; eluting with the mixture of methanol and water; vacuum concentrating the extracted liquid, and vacuum freeze drying to obtain product. The present invention has continuous production, high production efficiency and environment friendship.

Description

technical field [0001] The invention relates to a method for separating and purifying natural medicines, in particular to a method for separating and purifying aloe flavonoids by four-zone simulated moving bed chromatography. Background technique [0002] Aloe flavonoids can inhibit the activity of TPK and block the division of cancer cells. Aloe vera contains flavonoids, aloin, aloe polysaccharides and other impurities. The method of extracting aloe flavonoids is soaking, filtering and extracting to obtain crude products. However, to meet the medicinal requirements, it must be further purified. Currently, the commonly used purification method The column chromatography with neutral alumina as filler, although this method can be used for large-scale preparation, has low separation efficiency, serious waste of mobile phase, low utilization efficiency of stationary phase, low output, high cost, and low product purity. Can reach 60%, will reduce yield if will improve purity, ca...

Claims

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Application Information

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IPC IPC(8): C07D311/28C07H17/07A61K36/896
Inventor 张大兵王亚辉孟强王艳
Owner 江苏汉邦科技股份有限公司
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