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Quality control standard substance for microbe detection and its preparation method

A technology for microbial detection and control standards, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/inspection, etc., which can solve problems such as uncertainty in test results

Inactive Publication Date: 2007-12-12
杭州致远医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there is no unified standard substance for microbiological testing quality control, when microbiological testing laboratories of various systems and units test products such as microorganisms, there is no standard material for simultaneous testing and comparison, making the test results uncertain

Method used

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  • Quality control standard substance for microbe detection and its preparation method
  • Quality control standard substance for microbe detection and its preparation method
  • Quality control standard substance for microbe detection and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The microorganisms are adapted for 2 to 4 hours on the bacterial spore formation medium or fungal spore formation medium (the culture temperature of Bacillus subtilis var. The culture temperature of spores is 54°C to 56°C, and the culture temperature of Candida albicans is 25°C), the microorganisms enter the logarithmic growth phase, the number of microorganisms increases greatly, the microorganisms grow vigorously, the nutrients in the medium are exhausted, and the medium can be used The ingredients are greatly reduced, and the harmful substances produced are greatly increased. Microorganisms are cultured for more than 10 days in such a harsh environment where nutrients are insufficient and harmful substances exist, and more than 90% of microorganisms form spores and spores and enter a dormant state.

[0028] Wash the bacterial lawn on the culture medium with sterilized distilled water, mix thoroughly to form a 2.0×10 9 cfu / ml bacterial suspension, placed in a sterili...

Embodiment 2

[0095] Example 2 Used for synchronous positive control in the detection process of colony counting items

[0096] a. Take a "quality control standard substance for microbiological testing".

[0097] b, in the microbiological testing laboratory (biosafety protection laboratory). Open the plastic tube.

[0098] c. Take a pipette, draw 1.0ml of sterilized distilled water, add it into a plastic tube, and mix well.

[0099] d. Take another pipette, suck out the liquid in the plastic tube, and add it to the sterilized medium test tube (tube No. 1).

[0100] e. Continue to draw 1.0ml of sterilized distilled water, add it to the plastic tube, rinse the plastic tube, then suck out the sample liquid, and place it in the above-mentioned pilot test tube. Repeat this method 2 more times, fully wash the sample in the plastic tube, and place it in the pilot test tube.

[0101] f, continue to add sterilized distilled water into the test tube, the total amount is 5.0ml.

[0102] g, take a...

Embodiment 3

[0109] Example 3 Medium (nutrient agar) quality test

[0110] a. Take a "quality control standard substance for microbiological testing".

[0111] b. In the microbiological testing laboratory (biosafety protection laboratory). Open the plastic tube.

[0112] c. Take a pipette, draw 1.0ml of sterilized distilled water, add it into the plastic tube, and mix well.

[0113] d. Take another pipette, suck out the liquid in the plastic tube, and add it to the sterilized test tube.

[0114] e. Continue to draw 1.0ml of sterilized distilled water, add it to the plastic tube, rinse the plastic tube, then suck out the sample liquid, and place it in the above-mentioned pilot test tube. Repeat this method 2 more times, fully wash the sample in the plastic tube, and place it in the pilot test tube.

[0115] f, continue to add sterilized distilled water in the test tube, the total amount is 5.0ml, fully mix with a 1ml pipette (or with a mixer).

[0116] g. Use a 100ul pipette to absorb ...

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Abstract

The invention relates to a kind of microbiological standard matter that is used for quality control reference, microbiological checking experiment indication, and the preparation method. It comprises following steps: adding quantitative bacteria gemma or teleutospore with diluted required concentration into quantitative carrier that has been epoxyethane disinfected and is sterile, homogenizing, the bacteria gemma or teleutospore are combined on carrier homogeneously, putting said quantitative carrier into disinfected plastic pipe, and getting quality control standard substance for microbiological checking.

Description

technical field [0001] The invention relates to a quantitative microbial standard substance which is used as a quality control control in a microbiology testing laboratory to indicate the testing quality in the microbiology testing laboratory and a preparation method thereof. Background technique [0002] There are a large number of microorganisms (non-pathogenic microorganisms, pathogenic microorganisms, conditional pathogenic microorganisms, weakly toxic pathogenic microorganisms and highly toxic pathogenic microorganisms) in products (food, drinking water, cosmetics, medical supplies, daily necessities, hygiene products, etc.) disease microorganisms, etc.), because there are a large number of microorganisms in the product, most of them are propagules (microorganisms that are multiplying in large numbers), the product quality supervision and monitoring department and the internal laboratory of the enterprise conduct testing and testing, and the testing data can only represe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q3/00C12R1/01C12R1/645
Inventor 杨宁敏
Owner 杭州致远医学检验所有限公司
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