Unlock instant, AI-driven research and patent intelligence for your innovation.

Method of preparing cortex mori radicis extractive with glycosidase restraint function

A Morus alba extract and extract technology, applied in the field of traditional Chinese medicine extract and its preparation, can solve the problems of unsuitability for industrial production, complex preparation process, and low content of active ingredients

Active Publication Date: 2008-01-23
BEIJING WBL PEKING UNIV BIOTECH
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, among the extracts of Cortex Morus albicans, some preparation processes mainly extract flavonoids in Cortex Morus alba rather than alkaloids; some processes are too simple, resulting in low content of active ingredients, which must be taken in large quantities to be effective. efficiency; some preparation processes are too complicated, resulting in low yield of total alkaloids and high production costs, which are not suitable for industrialized production
However, the current research report does not involve the method of purifying the Morus alba extract with activated carbon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] After cutting 100g of Morus alba medicinal material, stir and extract three times at 35°C, the amount of water added is 8, 6, and 6 times the weight of the medicinal material, and the extraction time is 3, 2, and 1 hour respectively. The three extracts are combined, and the pressure is reduced at 60°C Concentrate, 200ml of the concentrated solution is centrifuged on an activated carbon column (activated carbon model is GH-15, the dosage is 100 grams, wet packing), the temperature of the upper column liquid is 50°C, and the activated carbon column is eluted with water until the washing liquid is clear (indrin Ketone reaction is negative) change to 15% ethanol for elution and start to receive the eluent, use 1-naphthol reagent to qualitatively detect the total sugar, end the elution when the Molish reaction disappears, 15% ethanol eluent 60 ° C Concentrated under reduced pressure, and the concentrated solution was dried under reduced pressure at 50° C. to obtain 3.5 g of C...

Embodiment 2

[0049] After cutting 100g of Morus alba medicinal material, stir and extract three times at 50°C, the amount of water added is 7, 5, and 5 times the weight of the medicinal material respectively, and the extraction time is 2, 1, and 1 hour respectively. The three extracts are combined and reduced at 60°C Concentrate, add 900ml of 95% ethanol to 300ml of the concentrated solution, place it overnight at room temperature, evaporate the ethanol from the supernatant, adjust it to 200ml of aqueous solution and put it on an activated carbon column (the model of activated carbon is GH-15, the dosage is 50 grams, and the column is wet-packed). The temperature of the upper column liquid is 30°C, and when the activated carbon column is eluted with water until the washing liquid is clear (ninhydrin reaction is negative), replace it with 20% ethanol for elution and start to receive the eluent, and use 1-naphthol reagent to qualitatively detect the total Sugar, when the Molish reaction disap...

Embodiment 3

[0051] 100g of Morus Alba medicinal material is cut, extracted three times with 30% ethanol under reflux, the amount of solvent is 7, 6, 5 times of the weight of medicinal material respectively, the extraction time is 2, 1, 1 hour respectively, and the three extracts are combined, Concentrate under reduced pressure at 60°C, centrifuge 300ml of the concentrated solution onto an activated carbon column (the type of activated carbon is GH-1, the dosage is 100 grams, wet packing), the temperature of the liquid on the column is 45°C, and the activated carbon column is eluted with water until the washing solution is clear When the ninhydrin reaction is negative, change to 30% ethanol for elution and begin to receive the eluate, use 1-naphthol reagent to qualitatively detect the total sugar, end the elution when the Molish reaction disappears, and 30% ethanol elution The solution was concentrated under reduced pressure at 60° C., and the concentrated solution was dried under normal pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a kind of Chinese traditional medicine extraction with function of restraining glycosidase. The detailed method is that: cut or crash the cortex mori radicis, extract efference after condensing the liquid deduced pressure, upper activated carbon pole at upper cleaning liquid or WAE after condensing the extraction as well as the upper activated carbon pole in water liquor form after removing the ethanol by taking the water or the ethanol solution as the extraction solvent. When the activated carbon pole is eluted into the clear shape, ethanol is used for eluting in replacement for the activated carbon pole and receiving the activated carbon pole eluting solution; inspect the total sugar with 1-naphthol reagent, stop the eluting when the reaction of the Molish disappears, then achieve the cortex mori radicis extraction after condensing and drying the ethanol eluting solution with deduced pressure.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a traditional Chinese medicine extract with glycosidase inhibitory effect and a preparation method thereof. Background technique [0002] Cortex Mori (CORTEX MORI) was first recorded in "Shen Nong's Materia Medica", listed as a middle grade, and has been recorded in all herbal medicines of all dynasties. Cortex Mori has diarrhea lung and relieves asthma, is sweet in taste and cold in taste, returns to the lung meridian, promotes diuresis and detumescence. Indications for asthma and cough due to lung heat, water retention in the lung, fullness and dyspnea, edema, beriberi, and dysuria. The use of mulberry bark to treat diabetes has long been recorded in ancient times: the usage of mulberry root soup recorded in volume 19 of "Qian Jin Yi" is: half a catty of mulberry root white bark (filed, fried) is coarse powder, and three coins per servi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/605A61P11/14A61P11/06A61K125/00
Inventor 段震文郭树仁樊利青何大林
Owner BEIJING WBL PEKING UNIV BIOTECH