Bacterium agent for renovation of organic pollution aquifer, producing and using method of the same

A technology of organic pollution and bacterial agent, applied in the field of microorganisms, can solve the problems of nitrobenzene polluted water, slow degradation of environmental pollutants, unable to continue to degrade pollutants, etc. Strong, continuous enhancement of the effect of water purification

Inactive Publication Date: 2008-04-02
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] On November 13, 2005, an explosion accident occurred in the aniline workshop of PetroChina Jilin Petrochemical Company's double-phenyl plant. On-site cleaning and pollution brought about during the cleaning process caused more than 100 tons of benzene pollutants (benzene, aniline and nitrobenzene) to flow into the Second Songhua River through East Line 10 of Jihua Company, causing major nitrobenzene pollution water event
However, it cannot fully meet the actual needs. This is because the pollutants scattered in the natural environment cannot be treated centrally and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The bacterial agent is composed of aerobic microorganisms: Bacillus, Arthrobacter, Aeromonas, Alealigenes, and Pseudomonas Bacillus (Pseudomonas), the parts by weight are 1:1:2:4:10, and the balance is the culture solution containing nitrobenzene.

[0023] Specifically: Bacillus megaterium, Arthrobacter simplex, Aeromonas hydrophila, Alealigenes eutrophus and Pseudomonas fluorescens.

[0024] The preparation method of bacterium agent: the aerobic microorganism that is 10% by weight percentage is inoculated in the Erlenmeyer flask that 150ml sterilized culture solution is housed, and the initial concentration of nitrobenzene reaches 20mg / L in the culture medium, and gauze pad is sealed, and is placed in 28 Shake culture in a constant temperature shaking incubator at ℃ for 5 days, then take out 1 / 3 of the culture solution, and then add the same amount of culture solution to make the initial concentration of nitrobenzene in the medium reach 20mg / L, and continue to culture ...

Embodiment 2

[0031]The bacterial agent is composed of aerobic microorganisms, and the aerobic microorganisms are: Bacillus megaterium, Arthrobacter simplex, Aeromonas hydrophila, Alealigenes eutrophus and Pseudomonas fluorescens (Pseudomonas fluorescens), the weight ratio is 1:1:2:6:12, and the balance is the culture solution containing nitrobenzene.

[0032] The culture solution is calculated by weight percentage: glucose 0.05%, peptone 0.02%, beef extract 0.03%, NaCl 0.3%, NaCl 2 HPO 40 0.3%, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O 0.02%, pH7.5.

[0033] The preparation method of bacterial agent: the bacterial agent of 5% by weight is inoculated in the Erlenmeyer flask that the sterilized culture fluid of 150ml is housed, and the initial concentration of nitrobenzene in the culture fluid is 80mg / L, and gauze pad is sealed, and is placed in 28 ℃ in a constant temperature shaking incubator for 4 days, then take out 1 / 5 of the culture solution, and then add the same amount of culture solutio...

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PUM

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Abstract

The invention relates to the microbial field, in particular to a microorganism agent for recovering the organic polluted water and preparation process and using method. The microorganism agent consists of aerobic microorganisms, in particular to pseudomonas fluorescens, alcaligeneseutrophus, aeromonas hydrophila, ArthrobacterSimplex and bacillus megaterium with the weight ratio is 1:1:1-4:2-6:3-12, and the residual is the culture liquid containing nitrobenzene. The degradation rate can reach between 97.7 percent and 100 percent when the invention is implemented in the polluted water containing nitrobenzene. The microorganism agent of the invention is made by that the nitryl aromatic hydrocarbon compound and the nutrition compound with relatively high concentration are acclimatized and sorted, and has strong growing and reproductive ability as well as adaptability; besides, the invention can assure the higher biomass in the environment at low cost, and does not cause secondary pollution and has the effect of continuously strengthening the purification of the water, ,thereby having good social, economic and ecological benefits.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent for repairing organically polluted water bodies and its preparation and use methods. Background technique [0002] my country's chemical and petrochemical industries have relatively obvious layout and structural environmental hazards. Many enterprise projects are placed in densely populated residential areas and upstream of drinking water sources along rivers, lakes and seas. A large amount of industrial waste is easily polluted. [0003] On November 13, 2005, an explosion accident occurred in the aniline workshop of PetroChina Jilin Petrochemical Company's double-phenyl plant. On-site cleaning and pollution brought about during the cleaning process caused more than 100 tons of benzene pollutants (benzene, aniline and nitrobenzene) to flow into the Second Songhua River through East Line 10 of Jihua Company, causing major nitrobenzene pollution water events. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/05
Inventor 李培军许华夏张春桂张海荣尹炜鞠京丽
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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