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Method for producing tangerine peel low methoxy group pectin by using pectinase

A technology of low methoxyl pectin and citrus peel is applied in the field of preparing low methoxyl pectin from citrus peel by using biological enzyme preparation, which can solve the pollution caused by acid-base waste environment, unstable product quality, and easy damage to equipment. Corrosion and other problems, to achieve good research and application prospects, easy process control, and low requirements for production conditions

Active Publication Date: 2011-01-19
HUNAN AGRI PRODS PROCESSING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods, they all have disadvantages such as high energy consumption, high pollution, unstable product quality, and long production cycle to varying degrees.
Taking the acid and alkali methods as an example, there are still defects such as difficult separation of residues, easy corrosion of equipment, high energy consumption, and a large amount of acid-base waste generated is likely to pollute the environment; and the shortcomings of the amidation method are also reflected in the amidation The effect will bring some harmful substances to the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The low-methoxyl pectin from Wenzhou citrus peel was prepared by the following method:

[0019] 1. Raw material pretreatment: use a pulverizer to crush 1Kg of dried Wenzhou satsuma citrus peels from which impurities and dirt have been removed into particles of 2mm to 3mm, soak them in deionized warm water at 70°C for 30 minutes to fully absorb water and soften them, and then Drain the wet mandarin orange peel and immerse it in boiling water for 8 minutes to inactivate the enzyme, put the enzyme-inactivated mandarin orange peel in 2500 mL of ethanol solution with a volume concentration of 95%, condense and reflux for 25 minutes, then centrifuge and dry to obtain the mandarin orange peel powder for use;

[0020] 2. Enzymatic hydrolysis and extraction: add 2000mL of water to the mandarin orange peel powder, then add 4.5g of pectinase (produced by Lihua Enzyme Preparation in Tianjin), stir evenly, at a temperature of 40°C and a pH value of 3.5 (pH value After adjusting and ...

Embodiment 2

[0027] The Jiangyong pomelo peel low-methoxyl pectin is prepared through the following method steps:

[0028] 1. Raw material pretreatment: crush 1Kg Jiangyong pomelo dry skin with a pulverizer into 2mm-3mm particles, soak in deionized warm water at 75°C for 35 minutes to fully absorb water and soften it, and then wet Jiangyong pomelo Drain the peel and immerse in boiling water for 10 minutes to inactivate the enzyme, place the deenzyme-inactivated Jiangyong pomelo peel in 3000 mL of 95% (volume concentration) ethanol solution to condense and reflux for 35 minutes, then centrifuge and dry to obtain Jiangyong pomelo peel powder for future use;

[0029] 2. Enzymatic hydrolysis extraction: Add 3000mL of water to Jiangyong pomelo peel powder, then add 6.0g of pectinase (produced by Tianjin Lihua Enzyme Preparation Factory), stir evenly, at a temperature of 40°C and a pH value of 3.5 (pH value After soaking for 3 hours under the condition of hydrochloric acid solution with a concen...

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PUM

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Abstract

The invention discloses a method for producing low-methoxy pectin of orange peel with the pectinase, which includes the following procedures that: the dried orange peel is shattered into granules, soaked with water to be softened, then undergoes enzyme inactivation, condensation and circumfluence, centrifuge, and spin drying, to obtain the powder of the orange peel, which is hydrolyzed by pectinase and centrifuged to obtain the extract of the pectin, which undergoes decolorating filtration to obtain the pectin filtrate, which is degreased and transformed with concentrated hydrochloric acid, filtered, rinsed and then filtered to obtain the filtered cake, which is shattered and dried to obtain the product of low-methoxy pectin. The method provided by the invention for producing the low-methoxy pectin has high extraction efficiency, low energy consumption, low environmental pollution, simple operation of the extraction, high productive efficiency and high quality of the product.

Description

technical field [0001] The invention relates to a method for preparing low-methoxyl pectin, in particular to a method for preparing citrus peel low-methoxyl pectin by using a biological enzyme preparation. Background technique [0002] Citrus is the fruit with the largest output in the world and the third largest agricultural product in international trade. In 2006, the world's annual output of citrus reached more than 120 million tons, accounting for about one-fifth of the world's annual fruit output; among them, my country's citrus output exceeded 16 million tons, ranking second in the world, and its planting area ranked first in the world. Whether citrus is eaten fresh or processed, the peel is its main by-product, accounting for about 20% to 40% of the fruit weight, and the pectin content in the peel is 20% to 30%, which is an important source of commercial low-methoxyl pectin. raw material. At present, except for a few people in China who use citrus peels for traditio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04
Inventor 单杨李高阳何建新苏东林张菊华张群付复华高川谭欢李志坚肖轲
Owner HUNAN AGRI PRODS PROCESSING INST