Method for producing tangerine peel low methoxy group pectin by using pectinase
A technology of low methoxyl pectin and citrus peel is applied in the field of preparing low methoxyl pectin from citrus peel by using biological enzyme preparation, which can solve the pollution caused by acid-base waste environment, unstable product quality, and easy damage to equipment. Corrosion and other problems, to achieve good research and application prospects, easy process control, and low requirements for production conditions
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Embodiment 1
[0018] The low-methoxyl pectin from Wenzhou citrus peel was prepared by the following method:
[0019] 1. Raw material pretreatment: use a pulverizer to crush 1Kg of dried Wenzhou satsuma citrus peels from which impurities and dirt have been removed into particles of 2mm to 3mm, soak them in deionized warm water at 70°C for 30 minutes to fully absorb water and soften them, and then Drain the wet mandarin orange peel and immerse it in boiling water for 8 minutes to inactivate the enzyme, put the enzyme-inactivated mandarin orange peel in 2500 mL of ethanol solution with a volume concentration of 95%, condense and reflux for 25 minutes, then centrifuge and dry to obtain the mandarin orange peel powder for use;
[0020] 2. Enzymatic hydrolysis and extraction: add 2000mL of water to the mandarin orange peel powder, then add 4.5g of pectinase (produced by Lihua Enzyme Preparation in Tianjin), stir evenly, at a temperature of 40°C and a pH value of 3.5 (pH value After adjusting and ...
Embodiment 2
[0027] The Jiangyong pomelo peel low-methoxyl pectin is prepared through the following method steps:
[0028] 1. Raw material pretreatment: crush 1Kg Jiangyong pomelo dry skin with a pulverizer into 2mm-3mm particles, soak in deionized warm water at 75°C for 35 minutes to fully absorb water and soften it, and then wet Jiangyong pomelo Drain the peel and immerse in boiling water for 10 minutes to inactivate the enzyme, place the deenzyme-inactivated Jiangyong pomelo peel in 3000 mL of 95% (volume concentration) ethanol solution to condense and reflux for 35 minutes, then centrifuge and dry to obtain Jiangyong pomelo peel powder for future use;
[0029] 2. Enzymatic hydrolysis extraction: Add 3000mL of water to Jiangyong pomelo peel powder, then add 6.0g of pectinase (produced by Tianjin Lihua Enzyme Preparation Factory), stir evenly, at a temperature of 40°C and a pH value of 3.5 (pH value After soaking for 3 hours under the condition of hydrochloric acid solution with a concen...
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