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Milk containing spleen polypeptide extraction and preparation method thereof

The technology of extract and spleen is applied in the field of peptide-containing milk and its preparation to achieve the effects of enhancing immunity, improving intestinal microecological environment and enhancing immune function.

Inactive Publication Date: 2008-04-30
山东银香大地乳业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is not yet a method to use spleen polypeptide extract as an active ingredient in health care products, combined with the special health functions of milk, to improve the immune function of the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Collect fetal bovine spleen tissue, wash fresh fetal bovine spleen tissue cells with ultrapure water at room temperature, add 4 times the volume of sterile ultrapure water to make a homogenate; then add 5 times the volume of sterile ultrapure water , mix well, freeze and thaw twice at -20°C; centrifuge the frozen-thawed homogenate at 4-8°C at 8,000 rpm for 20 minutes to remove the precipitate; trap the supernatant with a tangential flow ultrafiltration device , take the spleen polypeptide extract with a molecular weight of <30000 Daltons, wherein the content of polypeptides with a molecular weight of less than 90 Daltons is 48.0% to 55.5%, the content of polypeptides with a molecular weight of 90 to 130 Daltons is 10.1% to 10.2%, and the content of 130 to 130 Daltons The peptide content of 270 Daltons is 25.5% to 28.2%, and the content of peptides of 270 to 30,000 Daltons is 8.9 to 13.6%. It is obtained by sterilizing and filtering; adding 1ml of spleen polypeptide extra...

Embodiment 2

[0041]Collect fetal bovine spleen tissue, wash the frozen fetal bovine spleen tissue cells with ultrapure water at room temperature, add 5 times the volume of sterile ultrapure water to make a homogenate; then add 3 times the volume of sterile ultrapure water , mix well, freeze and thaw repeatedly at -30°C for 3 times; centrifuge the frozen-thawed homogenate at 4-8°C at 8,000 rpm for 10 minutes to remove the precipitate; use tangential flow ultrafiltration equipment to intercept the supernatant , take the spleen polypeptide extract with a molecular weight of <30000 Daltons, wherein the content of polypeptides with a molecular weight of less than 90 Daltons is 48.0% to 55.5%, the content of polypeptides with a molecular weight of 90 to 130 Daltons is 10.1% to 10.2%, and the content of 130 to 130 Daltons The peptide content of 270 Daltons is 25.5% to 28.2%, and the content of peptides of 270 to 30,000 Daltons is 8.9 to 13.6%; after sterilization and filtration, it is ready; add 0...

Embodiment 3

[0043] Collect fetal bovine spleen tissue, wash the fresh fetal bovine spleen tissue cells with ultrapure water at room temperature, add 3 times the volume of sterile ultrapure water to make a homogenate; then add 8 times the volume of sterile ultrapure water , mix well, freeze and thaw twice at -10°C; centrifuge the frozen-thawed homogenate at 4-8°C at 8,000 rpm for 30 minutes to remove the precipitate; trap the supernatant with a tangential flow ultrafiltration device , take the spleen polypeptide extract with a molecular weight of <30000 Daltons, wherein the content of polypeptides with a molecular weight of less than 90 Daltons is 48.0% to 55.5%, the content of polypeptides with a molecular weight of 90 to 130 Daltons is 10.1% to 10.2%, and the content of 130 to 130 Daltons The polypeptide content of 270 Daltons is 25.5% to 28.2%, and the content of polypeptides of 270 to 30,000 Daltons is 8.9 to 13.6%. It is obtained by sterilizing and filtering; adding 3ml of spleen polyp...

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PUM

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Abstract

The invention discloses a peptide-containing milk and process for preparation. The milk of the invention is processed through charging spleen polypeptide extract extracted from fetal bovine spleen tissue into common milk, and then processing through homogeneous process. During preparing the spleen polypeptide extract, steps of homogenate, freezing and thawing, interception, filtering and the like are employed, thereby processing spleen polypeptide extract with molecular weight < 30000 Daltons. A lot of experiments prove that the peptide-containing milk of the invention has the function of enhancing body immunity.

Description

technical field [0001] The invention relates to milk containing peptide and its preparation process, in particular to milk obtained by extracting spleen polypeptide extract from fetal bovine spleen tissue and its preparation process. Background technique [0002] People's modern tense and fast-paced lifestyle has invisibly promoted the occurrence of malignant tumors, hyperlipidemia, hypertension, arteriosclerosis, diabetes, obesity, etc. and the existence of a large number of sub-healthy people, and it also makes people pay more attention to their own health. . In addition to the treatment of the disease, more attention should be paid to the prevention and health care of the disease. Certain special functional foods can regulate body functions accordingly and can increase resistance to diseases. [0003] Milk is not only the most nutritious and one of the most commonly used foods, but also the best natural emulsion, which can be used as an ideal emulsion or nutritional for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/152A23L1/304A23L1/313A23L1/312A23L13/20A23L13/30
Inventor 陆家海谷登峰杜启科
Owner 山东银香大地乳业有限公司
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