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Cultivate method for cordyceps militaris link

A cultivation method, the technology of Cordyceps militaris, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of affecting the output, reducing the quality, improper deployment, etc., and achieve the effects of shortening the maturity cycle, improving labor efficiency and ensuring full absorption

Inactive Publication Date: 2010-07-21
袁有宝
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a uniform temperature is given to the above-mentioned period, or if the temperature is different, but it does not fully meet its growth needs, it will result in a decrease in the yield and quality of the fruiting bodies.
[0006] 2. The proportion of raw materials such as grains and water in the grain medium is improperly prepared
There are generally too many liquid strains in the existing medium, and too many liquid strains in the medium will cause excess nutrition on the surface of the medium, dense strains, and excessive growth, resulting in mycelium on the surface of the medium in a short period of time. Thickened mycelium, the thickened mycelium is pressed on the surface of the culture medium like a layer of quilt
Since the fruiting body is formed in the thinner mycelium on the surface of the medium, it is difficult for the thicker mycelium to produce sporocarps to form the fruiting body, so that the fruiting body is produced less and cannot reach the fruiting body on the surface of the medium full germination, and affect the subsequent yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, this embodiment includes the following steps:

[0038] a. Cultivate liquid strains;

[0039]According to the ratio of water to raw materials, prepare the medium according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.15%, vitamin B 10.005-0.010%, Pour the prepared liquid medium into the culture bottle for 121 ℃ high pressure steam sterilization for 30min, after cooling, insert the prepared pure strain with good stability and cultivate it for 5 days, which is the liquid strain.

[0040] b. The rice solid culture medium is prepared in the culture bottle;

[0041] The present invention selects a 500ml culture bottle, contains 33-38g of rice, 1-3g of silkworm chrysalis powder, and prepares the following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate diphosphate Potassium Hydrogen 1%-1.5%, Triamine Citrate 0.5%-1%, Vitamin...

Embodiment 2

[0056] Embodiment 2, this embodiment includes the following steps:

[0057] a. Cultivate liquid strains;

[0058] According to the ratio of water to raw materials, prepare the medium according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.1 5%, vitamin B 1 0.005-0.010%, pour the prepared liquid culture medium into the culture bottle for 120 ℃ high pressure steam sterilization for 29min, after cooling, insert the prepared pure strain with good stability and cultivate for 4 days, that is, the liquid strain.

[0059] b. The millet solid culture medium is prepared in the culture flask;

[0060] The present invention selects a 500ml culture bottle, contains 33-38g of millet, 1-3g of silkworm chrysalis powder, and prepares the following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate diphosphate Potassium Hydrogen 1%-1.5%, Triamine Citrate 0.5%-...

Embodiment 3

[0074] Embodiment three, the present embodiment is cultivated according to the following steps:

[0075] a. Cultivate liquid strains;

[0076] According to the ratio of water to raw materials, prepare the medium according to the following formula: glucose 5-6%, yeast extract 1-1.5%, peptone 1-1.5%, potassium dihydrogen phosphate 0.1-0.15%, vitamin B 1 0.005-0.010%, pour the prepared liquid culture medium into the culture bottle and carry out high pressure steam sterilization at 123°C for 31 minutes. After cooling, insert the prepared pure strain with good stability and cultivate it for 6 days, which is the liquid strain.

[0077] b. The corn solid medium is prepared in the culture bottle;

[0078] The present invention selects a 500ml culture bottle, contains 33-38g of corn, 1-3g of silkworm chrysalis powder, and prepares the following nutrient solution formula: glucose 2.5-3%, peptone 0.05%-1.5%, dipotassium hydrogen phosphate 0.2%-0.5%, diphosphate diphosphate Potassium Hy...

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PUM

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Abstract

The invention relates to a culturing method of Chinese Caterpillar Fungus, which is characterized in that: the grain culture mediums are put into a culturing house to culture after inoculation; the culturing process is divided into two periods, namely mycelium growing period and encarpium growing period. The invention has the advantages of providing an increasing-orderly temperature environment required for growing according to the growing features of the periods, therefore shortening the culturing cycle and improving the quality and output of the Chinese Caterpillar Fungus.

Description

technical field [0001] The invention relates to a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris is a precious Chinese medicinal material with high medicinal and nourishing effects. Wild Cordyceps is scarce and resources are extremely scarce. In order to meet the market demand, the existing artificially cultivated Cordyceps militaris is also known as Cordyceps militaris. Its active ingredients are close to natural, and the content of some components is even several times or dozens of times higher than that of natural Cordyceps. [0003] At present, many companies at home and abroad are engaged in the research of artificial cultivation of Cordyceps militaris, in order to obtain the best natural growth law of artificially cultivated Cordyceps, and strive to cultivate Cordyceps fruiting bodies with higher quality and higher yield, and a number of technologies have applied for patents Combining these technologies, it is not difficul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 孙婵娟袁博阳袁有宝
Owner 袁有宝