Method for rapidly breeding transgene abrotanum using micro adventive bud technique
A technology of transgenic and Artemisia annua, which is applied in the biological field, can solve the problems of long-term cultivation of pure varieties, restrictions on large-scale promotion of transgenic Artemisia annua, lack of genetic stability, etc., and achieve the effect of increasing the production of artemisinin
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Embodiment 1
[0021] Disinfection of Transgenic Artemisia annua Explants and Induction of Adventitious Buds
[0022] Young stem segments (1 cm long) containing growth points were cut from transgenic Artemisia annua plants as explants. Disinfect with 75% alcohol for 20 seconds, wash once with sterile water, 1.5% HgCl 2 Disinfect for 6 minutes and wash with sterile water 5 times. The sterilized transgenic Artemisia annua explants were transferred to the adventitious bud induction medium, which was MS medium+0.5mg / L 6-benzyladenine (6-BA)+0.05 mg / L naphthaleneacetic acid (NAA)+3% sucrose+0.65% agar powder. The culture temperature is 25±1° C., light is 16 hours a day, subculture is performed every 14 days, and a large number of microadventitious buds (length 1.5 cm) grow around the explant after 1 subculture. The induction rate of adventitious buds reached 91%.
[0023] Elongation and rooting of transgenic adventitious shoots of Artemisia annua
[0024] The explants with a large number of ...
Embodiment 2
[0028] Disinfection of Transgenic Artemisia annua Explants and Induction of Adventitious Buds
[0029] Young stem segments (1.5 cm long) containing growth points were cut from transgenic Artemisia annua plants as explants. Disinfect with 75% alcohol for 20 seconds, wash once with sterile water, 1.5% HgCl 2 Disinfect for 6 minutes and wash with sterile water 5 times. The sterilized transgenic Artemisia annua explants were transferred to the adventitious bud induction medium, which was MS medium+0.5mg / L 6-benzyladenine (6-BA)+0.05 mg / L naphthaleneacetic acid (NAA)+3% sucrose+0.65% agar powder. The culture temperature is 25±1° C., light is 16 hours a day, subculture is performed every 14 days, and a large number of microadventitious buds (length 1.5 cm) grow around the explant after 1 subculture. The induction rate of adventitious buds reached 94%.
[0030] Elongation and rooting of transgenic adventitious shoots of Artemisia annua
[0031] The explants with a large number o...
Embodiment 3
[0035] Disinfection of Transgenic Artemisia annua Explants and Induction of Adventitious Buds
[0036] Young stem segments (2 cm long) containing growth points were cut from transgenic Artemisia annua plants as explants. Disinfect with 75% alcohol for 20 seconds, wash once with sterile water, 1.5% HgCl 2Disinfect for 6 minutes and wash with sterile water 5 times. The sterilized transgenic Artemisia annua explants were transferred to the adventitious bud induction medium, which was MS medium+0.5mg / L 6-benzyladenine (6-BA)+0.05 mg / L naphthaleneacetic acid (NAA)+3% sucrose+0.65% agar powder. The culture temperature was 25±1° C., and the light was 16 hours a day. Subculture was performed every 20 days. After one subculture, a large number of microadventitious buds (1.5 cm in length) grew around the explants. The induction rate of adventitious buds reached 98%.
[0037] Elongation and rooting of transgenic adventitious shoots of Artemisia annua
[0038] The explants with a lar...
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