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Methods and means for protecting the skin against pathogenic microorganisms

A technology of pathogenic microorganisms and microorganisms, applied in the field of microorganisms, can solve the problem of high risk of Staphylococcus aureus infection

Active Publication Date: 2008-06-18
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been shown that in the first few days of life, this type of skin flora has not yet developed, so the risk of S. aureus infection is very high

Method used

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  • Methods and means for protecting the skin against pathogenic microorganisms
  • Methods and means for protecting the skin against pathogenic microorganisms
  • Methods and means for protecting the skin against pathogenic microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0557] Growth stimulation of Staphylococcus epidermidis in an in vitro well plate assay

[0558] Certain lactic acid bacteria have been identified in in vitro well plate assays to stimulate the growth of S. epidermidis on agar plates. These lactic acid bacteria are described here. To test this effect, pre-punched wells were filled with pre-cultured lactic acid bacteria and observed for growth stimulation of the indicator strain S. epidermidis. To increase the visual effect of growth stimulation, tellurite is used. Tellurite specifically stains staphylococci. Irritation was defined as the formation of a black ring around the wells filled with lactic acid bacteria and an increase in the number of colonies. data in figure 1 displayed in .

[0559] Cultivation and preparation of Lactobacillus:

[0560] Lactic acid bacteria were cultured from microcentrifuge tubes containing 1ml of MRS culture medium frozen at -80°C. Tubes were sealed and incubated at 37°C for 2 days. Tran...

Embodiment 2

[0574] Growth stimulation of Staphylococcus epidermidis in an in situ skin assay

[0575] Probiotic lactic acid bacteria have been identified as stimulating the growth of Staphylococcus epidermidis directly on the skin.

[0576] The culture of Staphylococcus epidermidis was diluted, applied directly to the skin, and allowed to air dry. An aliquot of lactic acid bacteria is then applied precisely to this area of ​​skin. Lactic acid bacteria can directly stimulate the indicator strain Staphylococcus epidermidis on the skin. After incubation, staphylococci were transferred from the skin to the agar plate using sticky tape. The agar plates were incubated at 37°C. An increase in the colony count indicates growth stimulation of the indicated strain on the skin ( figure 2 ). The Lactobacillus strains of the invention, in particular those deposited at the DSMZ, exhibit growth stimulation of the indicated strains described herein.

[0577] Cultivation and preparation of Lactobacil...

Embodiment 3

[0595] No Growth Stimulation of Staphylococcus aureus in In Situ Skin Assays

[0596] Using this assay it was possible to test whether Lactobacilli, which are able to stimulate the bacteria of the protective resident skin microflora, do not stimulate the bacteria of the unwanted transitory pathogenic flora. For this purpose, the indicator strain S. aureus was highly diluted and applied to the skin in the same way as for S. epidermidis (see Example 2). The stimulating activity of lactic acid bacteria was tested again. Stimulation of Staphylococcus aureus by the lactic acid bacteria was not observed. The Lactobacillus strains of the present invention, in particular those deposited at the DSMZ, do not show irritation to Staphylococcus aureus. data in image 3 given in.

[0597] Cultivation and preparation of Lactobacillus:

[0598] Lactic acid bacteria were cultured from microcentrifuge tubes containing 1ml of MRS culture medium frozen at -80°C. Tubes were incubated closed ...

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Abstract

Described are microorganisms which are, in a first aspect, able to to stimulate the growth of microorganisms of the resident skin microbial flora and which do not stimulate the growth of microorganisms of the transient pathogenic micro flora. In a second aspect microorganisms are described which are able to inhibit the growth of microorganisms of the transient pathogenic skin micro flora and which do not inhibit the growth of microorganisms of the resident skin micro flora. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

Description

technical field [0001] The present invention relates to microorganisms capable of stimulating the growth of resident skin microflora without stimulating the growth of transient pathogenic microflora. The invention also relates to compositions, such as cosmetic or pharmaceutical compositions, comprising such microorganisms, and to the use of such microorganisms in cosmetic, prophylactic or therapeutic applications. Background technique [0002] Human skin is inhabited by a large and diverse population of microorganisms that mainly live on the surface of the skin as commensal organisms in relatively stable compositions (Roth and James, 1988). This normal skin flora is called "intrinsic skin flora". The main function of human skin is to protect the underlying tissues against environmental influences (Feingold, 1985). Such normal skin flora especially protects the skin against possible invasion of pathogenic microorganisms (Bisno, 1984). Certain microorganisms predominate in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38A61K35/74A01N63/22A61K35/747
CPCA61K35/747A01N63/00C12R1/24C12R1/225A61K8/99A61Q19/00A61K35/74A61P17/00A61P17/04A61P17/06A61P17/14A61P17/16A61P31/00A61P31/02A61P31/04A61P31/22A61P33/00A61P37/08A01N63/22C12R2001/24C12N1/205C12R2001/225C12N1/20C12N1/38A61K9/0014A61K2035/11A61Q17/005
Inventor C·朗A·海尔曼M·维恩E·布德M·波特纳A·赖因德R·克内尔
Owner NOVO NORDISK AS