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Detecting and analyzing method for micro-element in galactophore tumour organization

A technology for breast tumors and trace elements, which is applied in the fields of analyzing materials, using wave/particle radiation for material analysis, measuring devices, etc., can solve the problem that the sensitivity of trace element detection and analysis is not very high, and achieve obvious guiding significance and high accuracy Effect

Inactive Publication Date: 2008-07-16
刘成林
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Problems solved by technology

[0004] The relative content of trace elements in normal breast tissue, benign breast tumor tissue and breast cancer tissue is different, but the traditional determination method is to use graphite furnace atomic absorption spectroscopy, etc., due to the low intensity of the light source, the sensitivity of trace element detection and analysis not very high

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Embodiment Construction

[0013] Concrete detection analysis method of the present invention is:

[0014] (1). Using sterilized medical equipment to remove a small sample from the patient's breast tumor;

[0015] (2). Rinse the sample with distilled deionized water and then fix it with 10% formalin buffer solution;

[0016] (3). The sample is subjected to X-ray fluorescence detection. The detection equipment is mainly composed of a double-knife slit, an ionization chamber, a sample stage, an optical microscope and an energy analysis system. The energy resolution of the energy analysis system (Model Link ISIS) produced by Oxford Instruments Company used in the detection is 134eV at 5.9keV. Adjust according to the energy of the monochromatic light required by the element being measured; the maximum spot size is 1×1.4mm 2 (During micro-area detection, the light spot is 50×50μm 2 ), the step size and scanning range of spot movement are determined according to the measurement accuracy requirements, and t...

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Abstract

The invention relates to a method for detecting and analyzing micro-elements in a breast tumor tissue and belongs to the technical field of micro-element detecting. The detecting and analyzing method of the invention is realized by using a disinfectant medical appliance for cutting off a micro-sample from the breast tumor of a patient; the sample is fixed by formalin buffer solution after being washed by distilled de-ionized water; X-ray fluorescence detection is carried out on the sample; an AXIL software is used for carrying out identification on the various micro-elements in the sample of the breast tumor on a computer; the computer is used for counting and analyzing the relative contents of the micro-elements and the values of Cu / Zn, Cu / Fe and Zn / Se in a breast cutting micro-sample and compared with the relative contents of the micro-elements and the values of Cu / Zn, Cu / Fe and Zn / Se in a normal breast cutting sample to conclude a diagnosing conclusion. By adopting the detecting and analyzing method, the method has the advantage and positive effect of high accuracy of a detecting result, thus being capable of achieving the goal of symptomatic treatment.

Description

Technical field [0001] The invention relates to a method for detecting and analyzing trace elements in breast tumor tissue, and belongs to the technical field of trace element detection. Background technique [0002] The X-ray fluorescence analysis method uses the molecules or atoms of a substance to be irradiated by an external light source, the molecules or atoms are excited, and when they de-excite, they emit fluorescence. The wavelength of fluorescence is related to the energy level structure of molecules or atoms, so the measurement of fluorescence The wavelength and intensity can determine the type and content of the element emitting the fluorescence, thereby determining the composition of the sample. In addition, the X-ray fluorescence analysis method can also be used to determine the trace elements and their contents in the sample. [0003] Synchrotron radiation is a high-intensity, broadband light source that enables synchrotron-radiation-based X-ray fluorescence a...

Claims

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Application Information

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IPC IPC(8): G01N23/223G06F17/00G06Q50/00
Inventor 刘成林
Owner 刘成林
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