Cigarette filter tip
A filter tip and cigarette technology, applied in the field of metallothionein, can solve problems such as short active lifespan, and achieve the effects of avoiding occurrence and development, avoiding damage, reducing aggregation and activation
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[0013] In order to better understand the essence of the present invention, the content of the invention is described below through examples and tests, but the protection scope of the present invention is not limited thereto. Those of ordinary skill in the technical field can realize the object of the present invention according to the contents disclosed in the present invention.
[0014] 1. Preparation and purification of metallothionein
[0015] (1) Construction of Escherichia coli engineering strains containing metallothionein gene by protein engineering method: Utilize molecular biology techniques to construct plasmid pGEM-MT and closed-circle plasmid pET-GST, then use restriction endonuclease BamH I, NheI Double-digested plasmid pGEM-MT and closed-circle plasmid pET-GST were separated by low-melting point agarose gel to recover MT and plasmid vector fragments, then ligated into PET-GST-MT with ligase, and transformed into Escherichia coli BI 21, The correctness of the pla...
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