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Cigarette filter tip

A filter tip and cigarette technology, applied in the field of metallothionein, can solve problems such as short active lifespan, and achieve the effects of avoiding occurrence and development, avoiding damage, reducing aggregation and activation

Inactive Publication Date: 2008-09-17
广州医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gas phase free radicals include alkoxy free radicals, alkane peroxy free radicals, etc., which have the characteristics of high activity and short life.

Method used

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Embodiment Construction

[0013] In order to better understand the essence of the present invention, the content of the invention is described below through examples and tests, but the protection scope of the present invention is not limited thereto. Those of ordinary skill in the technical field can realize the object of the present invention according to the contents disclosed in the present invention.

[0014] 1. Preparation and purification of metallothionein

[0015] (1) Construction of Escherichia coli engineering strains containing metallothionein gene by protein engineering method: Utilize molecular biology techniques to construct plasmid pGEM-MT and closed-circle plasmid pET-GST, then use restriction endonuclease BamH I, NheI Double-digested plasmid pGEM-MT and closed-circle plasmid pET-GST were separated by low-melting point agarose gel to recover MT and plasmid vector fragments, then ligated into PET-GST-MT with ligase, and transformed into Escherichia coli BI 21, The correctness of the pla...

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PUM

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Abstract

The invention discloses a cigarette filter tip, comprising metallothionein. The cigarette filter tip has the advantages of eliminating the gas-phase free radicals in the cigarette flue gas, guarding against damage of cell ingredient and occurring and developing of diseases caused by the gas-phase free radicals in the cigarette flue gas, reducing the aggregation and activation of macrophage in the lung, and guarding against severe damage caused by respiratory burst and releasing active oxygen free radicals.

Description

technical field [0001] The present invention relates to the use of metallothionein, in particular to the application of metallothionein in preparing cigarette filters. The invention also relates to a cigarette filter, in particular to a cigarette filter capable of reducing free radicals in the gaseous phase of cigarette smoke. Background technique [0002] Cigarettes contain a large number of harmful free radicals, including solid-phase free radicals and gas-phase free radicals. Solid-phase free radicals include polycyclic aromatic hydrocarbon free radicals and quinone / semiquinone free radicals, which have the characteristics of long life and stable properties, and can be intercepted by biological filters. The gas phase free radicals include alkoxy free radicals, alkane peroxy free radicals, etc., which have the characteristics of high activity and short life. Gas-phase free radicals can damage cell components, leading to the occurrence and development of diseases. At the ...

Claims

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Application Information

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IPC IPC(8): A24D3/06
Inventor 李冰谭洁莹曹晓敏陈祯付欣刘启才彭燕
Owner 广州医学院
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