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Avermectin and erythromycin synthesis gene cluster fused combination biological synthesis application

A technology of abamectin and biosynthesis, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of abamectin having no antibacterial activity and the like

Inactive Publication Date: 2008-09-17
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Abamectin has no antibacterial activity against bacteria and fungi

Method used

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  • Avermectin and erythromycin synthesis gene cluster fused combination biological synthesis application
  • Avermectin and erythromycin synthesis gene cluster fused combination biological synthesis application
  • Avermectin and erythromycin synthesis gene cluster fused combination biological synthesis application

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Experimental program
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Embodiment Construction

[0042] Embodiment with reference to accompanying drawing

[0043] Figure 1 is a schematic diagram of the procedure for producing novel antibiotic structural analogs according to the present invention.

[0044] A and B: respectively, two gene fragments upstream of the abamectin synthesis gene cluster. The inactivation of this gene will not affect the main functions such as the growth and development of the strain and the production of antibiotics. For the insertion of the kana resistance gene in the genome.

[0045] C: a segment of gene downstream of the abamectin synthesis gene cluster. Used for homologous exchange between genomes during cell fusion.

[0046] D, E: two gene fragments downstream of the erythromycin synthesis gene cluster, respectively. The inactivation of this gene will not affect the main functions such as the growth and development of the strain and the production of antibiotics. Used for the insertion of the ampicillin resistance gene as well as the "C"...

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Abstract

The invention relates to the application of combinatorial biosynthesis which combines avermectin and erythrocin to synthesize a gene cluster. The following genes are utilized to encode the novel biosynthesis of an antibiotic avermectin structure analog: 1) aver-1:504bp and aver-2:507bp, aver-C:651bp, Kan:1278bp, ery-ORF6:501bp and ery-ORF7:438bp; Amp:861bp. Antibiotic markers are made for the synthetic gene cluster upstream or downstream of different polyketone compounds so as to get the structure analog of antibiotic avermectin through cell fusion technology. The invention provides a method for producing the novel microorganism of the antibiotic avermectin structure analog. The method can be used for searching for and finding out compounds, antibiotics, genes or protein used in medicine, industry or agriculture, and also provides new technology and means for the high production of bioactive molecule and creation of new microbial pesticide.

Description

technical field [0001] The invention relates to combinatorial biosynthesis, in particular to an application of combinatorial biosynthesis fused with avermectin and erythromycin synthetic gene clusters. The upstream or downstream of the synthetic gene clusters of different polyketides are marked with antibiotics, and the structural analogs of abamectin can be obtained through cell fusion technology. The present invention provides a microbial method for producing novel antibiotic structural analogs, which can be used to search for and discover compounds, antibiotics, genes or proteins that can be used in medicine, industry or agriculture. Background technique [0002] Combinatorial biosynthesis is a method of targeted manipulation of certain genes to obtain a large number of "unnatural" natural compounds, and its research is based on the biosynthetic pathways of various compounds in organisms It is one of the new technologies in the development of new microbial drugs. Combin...

Claims

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Application Information

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IPC IPC(8): C12N15/31C12N15/63C12N15/09C12P19/62
Inventor 乔建军黄惠娟孙艳华范凡兰玉
Owner TIANJIN UNIV
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