Multiple PCR fast detecting method for oral cavity pathogen

Abstract

The invention relates to a multiple PCR rapid detection method of common pathogen in mouth such as Actinobacillus actinomycetemcomitans, Bacterides forsythus, F. nucleatum and Porphyromones gingivalis and belongs to the field of molecular biology technology. The invention utilizes the multiple PCR to increase specific gene of Actinobacillus actinomycetemcomitans, Bacterides forsythus, F. nucleatum and Porphyromones gingivalis in mouth and utilizes electrophoresis to detect the specific gene. By designing the primers of Actinobacillus actinomycetemcomitans, Bacterides forsythus, F. nucleatum and Porphyromones gingivalis, the multiple PCR rapid detection method has the advantages of the uniform reaction condition, the sensitivity and the strong specificity of the method, the simultaneous detection and identification of the four bacteria in the specimen, accurate judgment of infection of different bacteria and greatly promoting the detection speed and the detection efficiency.

Description

technical field [0001] The invention relates to a multiplex PCR rapid detection method for common oral pathogens Actinobacillus actinomycetes, Bacteroides forsei, Fusobacterium nucleatum and Porphyromonas gingivalis, belonging to the technical field of molecular biology. Background technique [0002] There are about 500 kinds of bacteria living in the human oral cavity, and about 300 of them grow in the subgingival environment. Studies have shown that almost all types of periodontal disease are caused by bacterial infection of periodontal plaque, but only a few bacteria are caused by Alone or synergistically cause the destruction of periodontal tissue, the toxic products produced by bacteria and their metabolism, including bacterial antigenic components, various enzymes, toxins and many metabolites can directly stimulate and destroy periodontal tissue, or cause local immunity of periodontal tissue Reaction, causing tissue damage, which is the main cause of periodontal diseas...

AI Technical Summary

Problems solved by technology

There have been literature reports on the use of PCR technology to detect pathogenic bacteria separately, which has been welcomed by the clinic. In order to further improve the detection efficiency, multiplex PCR technology was used to amplify four kinds of bacteria at the same time. However, according to general principles, in the PCR reaction, different primers Different reaction conditions need to be selected to optimize the PCR reaction. In the same reaction, the more primers are added, the more difficult it is to choose suitable reaction conditions.

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