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Preparation of microballoons based on microfluid

A technology of microfluidics and microfluidic channels, which is applied in the direction of microsphere preparation, microcapsule preparation, liquid separation into beads and granulation, etc. It can solve the problems of high production equipment requirements, high production cost, uneven particle size, etc., and achieve convenient The effect of integration and miniaturization, small liquid requirement, and low preparation cost

Inactive Publication Date: 2008-10-08
NANJING DONGJIAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used polymer microsphere preparation methods include emulsion polymerization, dispersion polymerization, seed polymerization, suspension polymerization and microsuspension polymerization, etc., but the particle size of microspheres prepared by this method is in the nanometer and submicron range, and will Difficulty en

Method used

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  • Preparation of microballoons based on microfluid
  • Preparation of microballoons based on microfluid

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] The microfluidic channel system is used to make the colloidal solution or the precursor solution form droplets in the mobile phase, and polymer microspheres for biological analysis and protein, gene, and drug screening carriers are formed based on the droplet template. The specific preparation method includes the following steps :

[0027] Preparation of microfluidic channels: use micromachining technology to build a microfluidic channel network, or choose a needle, polymer tube, or tee to connect a T-shaped channel with two inlets, namely the dispersed phase inlet 11 and the continuous phase inlet 12. There is 1 exit 13;

[0028] Preparation of the droplet template: the dispersed phase and the continuous phase two-phase solution are respectively loaded into the syringes, connected to their respective inlets, and the flow rate of the two-phase solution is controlled by a digitally controlled syringe pump to obtain a uniform and stable droplet template from the outlet 13;

...

Example Embodiment

[0035] Example 1: Preparation of polystyrene microspheres

[0036] 1. Preparation of the channel:

[0037] ① Choose three Teflon tubes with a length of 10cm and an inner diameter of 500μm as the channels for the continuous phase, the dispersed phase and the outlet respectively. One end of the continuous phase pipeline is inserted with a 6-gauge needle as the inlet of the polyvinyl alcohol solution; one end of the dispersed phase is inserted with a 6-gauge needle as the inlet of the polystyrene solution, and the other end is inserted with a 60μm inner diameter and 150μm outer diameter needle as polystyrene Outlet of ethylene solution.

[0038] ② Connect the above three Teflon tubes with a Peek tee with an inner diameter of 500μm. The outlet needle of the dispersed phase channel is located on the central axis of the outlet tube, and the continuous phase channel is perpendicular to the other two channels.

[0039] 2. Preparation of droplet template:

[0040] ①. Dissolve polystyrene s...

Example Embodiment

[0047] Example 2: Preparation of silica colloidal crystal microspheres

[0048] 1. Preparation of the channel:

[0049] ①. Three Teflon tubes with a length of 10cm and an inner diameter of 500μm are used as channels for the continuous phase, the dispersed phase and the outlet respectively. One end of the continuous phase pipeline is inserted with a 6-gauge needle as the inlet of the polyvinyl alcohol solution; one end of the dispersed phase is inserted with a 6-gauge needle as the inlet of the polystyrene solution, and the other end is inserted with a 110μm inner diameter and 300μm outer diameter needle as polystyrene Outlet of ethylene solution.

[0050] ② Connect the above three Teflon tubes with a Peek tee with an inner diameter of 500μm. The outlet needle of the dispersed phase channel is located on the central axis of the outlet tube, and the continuous phase channel is perpendicular to the other two channels.

[0051] 2. Preparation of droplet template:

[0052] ①. Disperse ...

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Abstract

A microfluid-based microballoon preparation method adopts a microfluid channel system to lead a colloidal solution or a pre-polymer solution to form liquid drips in a mobile phase. The formation of the liquid drip-based template is used for bioanalysis and polymeric micro spheres of protein, gene and drug screening carrier. The preparation method includes the following steps: preparation of the microfluid channel: establishing a microfluid channel network by adopting the micro-machining technology or connecting a T-shaped channel with a pinhead, a polymer pipe and a tee joint (the channel is provided with two inlets, namely, a dispersed phase inlet and a continuous phase inlet and an outlet); preparation of the liquid drip template: encasing the two phase solutions of a dispersed phase solution and a continuous phase solution respectively into a pin case, connecting with the respective inlet, digital controlling an ejector jet pump to control the velocity of flow of the two phase solutions and acquiring even and stable liquid drip template from the outlet; preparation of the microballoon: drying and solidifying the liquid drip template and removing the impurities on the surface or inside to acquire a polymer microballoon according with the requirement of bioanalysis and protein, gene and drug screening carrier.

Description

technical field [0001] The invention relates to a method for preparing microspheres based on microfluidics. In the preparation method, liquid droplets are formed through the shear force and surface tension of the continuous phase liquid on the dispersed phase liquid, and then solidified and dried to form polymer microspheres that meet the carrier requirements of biological analysis and protein, gene, drug screening, etc. Background technique [0002] With the continuous development of biomedical technology, liquid-phase chip technology with microspheres as the carrier has been more and more used in biological analysis and protein, gene, and drug screening. Compared with other forms of solid phase carriers, microspheres have significant advantages: First, the specific surface area of ​​microspheres is large, which can increase the ratio of effective reaction surface area to volume, so the chemical reaction on the surface can be carried out in a smaller volume ; Second, using...

Claims

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Application Information

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IPC IPC(8): B01J13/02B01J2/02
Inventor 顾忠泽赵远锦赵祥伟扈靖
Owner NANJING DONGJIAN BIOLOGICAL TECH
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