Large scale preparation method of microbe microcapsules

A technology of microcapsules and microorganisms, which is applied in the direction of microcapsule preparations and microsphere preparations, can solve the problems of low yield of high-quality microcapsules, loss of microbial cell activity, and large number of microbial cells, and achieve low cost of materials and equipment, Effects of reduced utilization and controllable cell density

Inactive Publication Date: 2008-10-22
ACAD OF STATE GRAIN ADMINISTRATION
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Problems solved by technology

However, the number of microbial cells required for the post-fermentation coating method is huge, and the presence of a large number of cells reduces the yield of high-quality microcapsules
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Materials:

[0023] 1. Sodium alginate solution, 1.5% w / v.

[0024] 2. CaCl 2 Solution, 0.1mol / L.

[0025] 3. Enterococcus faecium strains, on May 22, 2008, were preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee (Address: Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, postal code 100101), it is recommended The classification name of the species is Enterococcus Faecium, and the preservation number is CGMCC No.2516.

[0026] 2. Method

[0027] 1. Preparation of strain seed liquid: inoculate the preserved Enterococcus faecium strain in liquid medium, and incubate at a constant temperature of 37±1°C for 8-10 hours. When the number of viable bacteria reaches the logarithmic growth phase, the density is about 10 7 -10 8 End the culture at cfu / ml and keep it for later use.

[0028] 2. Preparation and sterilization of capsule material solution: Add sodium algina...

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Abstract

The invention provides a method for large-scale preparation of micro-organism microcapsules, belonging to the technical field of micro-organism and biology. The method of the invention comprises the steps as follows: (1) capsule solution preparation: preparing the sodium alginate solution with the mass volume specific concentration of 1-2% w/v and carrying out the operation of sterilization; (2) preparation of curing agent: preparing the CaCl2 solution of 0.1-0.3mol/L and sterilizing; (3) preparation of the micro-organism microcapsules: micro-organism seed liquid with the logarithmic growth phase is added into the sterilization capsule solution till that the density of the solution is about 10<6>cfu/ml; the solution is then injected into the aseptic CaCl2 solution through the aseptic filter film and a nozzle by high-pressure air, then mixed and solidified till that supernatant is precipitated so as to gain the microcapsule and to wash the capsule; (4) after-covering culture: the microcapsules covered by micro-organism cells are arranged in fermentation broth and cultured till logarithm growth telophase, so as to gain the micro-organism microcapsule by filtration and separation. The method of the invention has the advantages of overcoming the defect that the micro-organism microcapsule which is prepared by covering-method before the sterilization can not be produced in large-scale, large production, simple operation and low cost of material and equipments.

Description

technical field [0001] The invention relates to a method for preparing microbial microcapsules in large quantities, and belongs to the field of microorganisms and biotechnology. Background technique [0002] In the field of biotechnology, microcapsule technology is formed with the development of immobilized cell technology, that is, the method of forming microcapsules is used to embed biological substances such as cells. The advantages of microcapsule immobilized microbial cells are as follows: 1) A tiny culture environment is formed during cell culture, which reduces the influence of shear force on cells during culture, so that operations such as stirring and direct ventilation will not affect the stability of cells. Normal growth increases cell density and allows repeated and continued use of cells without substantial loss. 2) The microcapsule can trap the target product in the membrane, and the product can be separated from the culture solution through the separation of ...

Claims

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Application Information

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IPC IPC(8): B01J13/04
Inventor 綦文涛郝淑红王婷婷张晓琳郭伟群李爱科
Owner ACAD OF STATE GRAIN ADMINISTRATION
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