Method for preparing donkey-hide gelatin oligopeptide
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A technology of donkey-hide gelatin and mucilage, which is applied in the field of extraction technology of medicinal effect substances of traditional Chinese medicine, can solve the problems of increasing gastrointestinal burden, failing to achieve medicinal effect, poor digestion ability, etc. Effect
Inactive Publication Date: 2008-10-29
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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However, collagen accounts for 80% of donkey-hide gelatin, which is difficult to digest and absorb, especially for patients with weak spleen and stomach, the activity of enzymes in the digestive tract is
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Embodiment 1
[0030] Raw materials: Ejiao 300g,
[0031] step
[0032] 1. Grind donkey-hide gelatin, add 20 times the amount of water (g / g), heat to dissolve, let it stand, remove the upper layer of fat, adjust the pH value to 2.0±0.1, add pepsin, and enzymolyze it in a water bath at 41±0.5°C for 2 hours, then Inactivate in a water bath at 85±0.5°C for 15 minutes, let cool; adjust the pH value to 8.0±0.1, add trypsin, enzymolyze in a water bath at 41±0.5°C for 3 hours, then inactivate in a water bath at 85±0.5°C for 15 minutes, put cold.
[0033] 2. Put the enzymatic solution in a water bath and heat it to 40°C, then pass it through an ultrafiltration column with a molecular weight cut-off value of 3kD or >5kD, collect the ultrafiltrate, and separate it with a macroporous adsorption resin column to remove sugars and other impurities , and then get 3kD or 5kD peptide components. Carry out 2 enzymatic hydrolysis, ultrafiltration, and separation on the primary water wash solution greater th...
Embodiment 2
[0036] Raw materials: 300ml of mucilage in donkey-hide gelatin production,
[0037] step
[0038] 1. Donkey-hide gelatin glue, add 15 times the amount of water (v / v), adjust the pH value to 2.0±0.1, add pepsin, enzymolyze it in a water bath at 41±0.5°C for 2 hours, and then inactivate it in a water bath at 85±0.5°C for 15 minutes , let cool; adjust the pH value to 8.0±0.1, add trypsin, enzymatically hydrolyze in a water bath at 41±0.5°C for 3 hours, then inactivate in a water bath at 85±0.5°C for 15 minutes, and let cool.
[0039] 2. Put the enzymatic solution in a water bath and heat it to 40°C, then pass it through an ultrafiltration column with a molecular weight cut-off value of 3kD or >5kD, collect the ultrafiltrate, and separate it with a macroporous adsorption resin column to remove sugars and other impurities , and then get 3kD or 5kD peptide components. Carry out 2 enzymatic hydrolysis, ultrafiltration, and separation on the primary water wash solution greater than ...
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Abstract
The invention provides a method for preparing donkey hide gelatin oligo-peptide and relates to a process for extracting pharmacodynamic substance from traditional Chinese medicine. The protein in the medicine normally can be absorbed and utilized by an organism only after being decomposed into the oligo-peptide or amino acid by the digestive juice enzyme. The collagen protein, which accounts for 80% of the content of the donkey hide gelatin, is difficult to be digested and absorbed, so that the due drug effect can not be produced. The method adopts the bionic principle and technology, i.e. extracting the pharmacodynamic substance of the oligo-peptide from the donkey hide gelatin by using the biological enzymolysis technology and the ultrafiltration technology. First, pepsin and trypsinase are added to the raw medicine of the donkey hide gelatin for hydrolyzation at a certain pH value and a certain temperature; then, ultrafiltration is conducted by using a 3kD or 5kD ultrafiltration column to obtain an ultrafiltrate, which is then separated and purified by using a macroporous resin column to further obtain a donkey hide gelatin oligo-peptide eluate; finally, the obtained donkey hide gelatin oligo-peptide eluate is decompressed, concentrated at a low temperature and dried to become the finished product of donkey hide gelatin oligo-peptide, which is verified by the pharmacodynamic test, has the efficacies of increasing the blood, enhancing the immunity of the organism, anti-aging, etc., and meanwhile provides the process and raw material for preparing other oral liquids and injections.
Description
technical field [0001] The invention relates to an extraction process of effective substances of traditional Chinese medicines. Background technique [0002] Proteins in food or medicines are generally enzymatically hydrolyzed into low peptides or amino acids by the digestive juices, and then absorbed and utilized by the body to exert nutritional and medicinal effects. Donkey-hide gelatin is a well-known traditional Chinese medicine. Its taking method is to dissolve it, and to make ointment with different formulas, all of which are administered orally. However, collagen accounts for 80% of donkey-hide gelatin, which is difficult to digest and absorb, especially for patients with weak spleen and stomach, the activity of enzymes in the digestive tract is low, and their digestibility is poor. At the same time, it will increase the burden on the stomach and intestines. Also there is no other technology to improve this shortcoming of donkey-hide gelatin at present. The inventi...
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