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Mangrove plant growth promoting azotobacter (DZY-X1) and use thereof

A technology for promoting rhizosphere growth and mangrove plants. It is applied in the directions of bacteria, microorganisms, and microorganism-based methods. It can solve the problems of low survival rate and achieve the effect of improving productivity and promoting growth.

Inactive Publication Date: 2008-11-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the survival rate of introduced strains in our country's mangroves is low, and there are also various hidden dangers caused by foreign species invasion

Method used

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  • Mangrove plant growth promoting azotobacter (DZY-X1) and use thereof
  • Mangrove plant growth promoting azotobacter (DZY-X1) and use thereof
  • Mangrove plant growth promoting azotobacter (DZY-X1) and use thereof

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Experimental program
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Effect test

Embodiment Construction

[0032] 1. Materials and methods

[0033] 1. Materials

[0034] 1.1 Soil sample collection

[0035] The samples were collected from the rhizosphere sediments of Bruguiera gymnorhiza in the mangrove area along the Hongsha River in Sanya City, Hainan Province. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory for low temperature storage.

[0036] 1.2 Medium

[0037] Ashby Broth: Mannitol 10.0g, KH 2 PO 4 ·H 2 O 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, CaSO 4 0.1g, CaCO 3 5.0g, 1000mL deionized water, pH 7.0.

[0038] Ashby solid medium: Ashby liquid medium + 2.2% agar.

[0039] to improve Nitrogen-free liquid medium: sucrose 10g, malic acid 5.0g, K 2 HPO 4 ·H 2 O 0.1g, KH 2 PO 4 ·H 2 O0.4g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, NaCl 2 MOO 4 ·H 2 O 0.002g, FeCl 3 0.01g, 1000ml deionized water, pH 7.0.

[0040] LB medium: yeast extract 5g, tryptone 10g, NaCl 10g, agar 1-2%, deionized water ...

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Abstract

The invention provides a mangrove plant rhizosphere growth-promoting nitrogen fixing bacterium (DZY-X1) and an application thereof. The bacterium is the vibrio sp. 2006-DZY-X1 screened from the rhizosphere settlings of the tropical mangrove plants and contains 16SrDNA serial and nifH and is stored in the CCTCC; the collection number is CCTCCNO: M207118. The strain has high biological nitrogen fixing activity; the microbial inoculum with the bacterium is used to breed mangrove plants such as rhizophora stylosa and bruguiera, which can evidently promote the growth of plants, thereby being capable of preparing the biological nitrogen fixing bacterium fertilizer promoting the growth of mangrove plants.

Description

technical field [0001] The patent application belongs to the field of biotechnology, and in particular relates to a mangrove rhizosphere nitrogen-fixing bacterium with good growth-promoting effect on mangrove plants and its application for preparing biological nitrogen-fixing bacterial fertilizer. Background technique [0002] Mangroves are woody plant communities naturally distributed in the intertidal zone of tropical and subtropical coasts, covering about 60-75% of tropical and subtropical coasts, playing an important role in maintaining the ecological balance of bays and estuaries, and are an important part of marine productivity (Holguin et al., 2001), considered to be a highly productive ecosystem. The ecosystem has high levels of organic matter (100 TgC per year in global litter), providing organic matter to adjacent coastal waters and associated biome habitats (Holguin et al., 2001; Lugomela and Bergman, 2002). However, mangrove ecosystems are poor in inorganic nutr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/63
Inventor 董俊德张燕英杨志浩杨斌张偲王友绍孙翠慈
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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