Method for stabilization of biological molecule and composition

A technology of biomolecules and compositions, applied in the field of enzymes or labeled antibodies, and compositions containing enzymes or labeled antibodies, can solve problems such as increased cost of enzymes or labeled antibodies, and achieve the effect of increasing stability

Inactive Publication Date: 2008-11-12
TOYOBO CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in many cases, such methods cannot fundamentally solve the problem, and the expense of using enzymes or labeled antibodies increases

Method used

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  • Method for stabilization of biological molecule and composition
  • Method for stabilization of biological molecule and composition
  • Method for stabilization of biological molecule and composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] 1 kg of cocoon silk (produced by Bombyx mori) was subjected to hot water treatment in 50 L of 0.2% aqueous sodium carbonate solution (pH 11-12) at 95° C. for 2 hours to extract sericin hydrolyzate. The obtained sericin hydrolyzate extract was filtered through a filter with an average pore diameter of 0.2 μm to remove aggregates. The filtrate was then desalted through a reverse osmosis membrane to obtain a 0.2% aqueous solution of clear and colorless sericin hydrolyzate.

[0101] The solution was concentrated to a concentration of about 2% with an evaporator, and then freeze-dried to obtain 100 g of sericin hydrolyzate in powder form with a purity of 90% or more and an average molecular weight of 20,000.

[0102] Example 1

[0103] The reagent solution described below containing peroxidase (Toyobo, PEO-302) and sericin hydrolyzate was stored at 25°C for 2 months or at 50°C for 7 days, and the remaining activity ratio (activity after storage) was determined. Ratio to th...

Embodiment 2

[0116] To the reagent solution containing peroxidase and 2 g / L sericin hydrolyzate prepared in Example 1, 0.5 g / L sodium azide was added as a preservative to prepare a reagent solution. The reagent solution was then stored at 25°C for 14 days, and the remaining activity ratio (ratio of activity after storage to activity immediately after preparation) was measured.

Embodiment 3

[0124] The reagent solution described below containing lipoprotein lipase (Toyobo, LPL-311) and sericin hydrolyzate was stored at 25° C. or 40° C. for 14 days, and the remaining activity ratio (activity after storage versus that at that time after preparation) was determined. active ratio).

[0125] Preparation of reagents

[0126]Each reagent having the following composition was prepared.

[0127] PIPES-NaOH 50mM pH 7.0

[0128] Triton X-100 1g / L

[0129] Lipoprotein lipase (Toyobo, LPL-311) 5,000U / L

[0130] Sericin hydrolyzate 0.2 to 2g / L

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PUM

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Abstract

The object is to provide a method for stabilization of a biological molecule and a composition, specifically a method for stabilization of an enzyme or a labeled antibody for use in a clinical diagnosis and a composition. Thus, disclosed is a method for stabilization of a biological molecule which is characterized by allowing (a) the biological molecule and (b) sericin and / or a hydrolysate or equivalence thereof to coexist with each other. Also disclosed is a composition having a biological molecule stabilized therein, which is characterized in that the components (a) and (b) coexist with each other in the composition. Further disclosed is a composition for stabilizing a biological molecule, which comprises sericin and / or a hydrolysate or equivalence thereof.

Description

technical field [0001] The present invention relates to methods for stabilizing biomolecules and compositions containing biomolecules, in particular, methods for stabilizing enzymes or labeled antibodies used in clinical diagnosis and compositions containing enzymes or labeled antibodies. technical background [0002] Enzymes, and labeled antibodies produced by modifying antibodies with enzymes using various compounds, are widely used due to their high substrate specificity and ease of manipulation. For example, they can be used for the production of analytical reagents for molecular biology, analytical reagents for biochemical purposes, in vitro diagnostic reagents, liquid in vitro diagnostic reagents, dry in vitro diagnostic reagents in the form of chips or crumbs, enzyme sensors or enzyme electrodes, pharmaceuticals , food, drink, etc. Enzymes used to produce the above compositions can be labeled with labeling compounds such as dyes, biotin, and avidin, modified with var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C07K1/00C07K16/00G01N33/531
CPCC07K16/00C12N9/96G01N33/531
Inventor 岸本高英梶谷香代子梶谷和生山田英幸佐佐木真宏
Owner TOYOBO CO LTD
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