Method for prepraring medicine carrying chitosan neural regeneration tube with controlled degradation

A technology of nerve regeneration and chitosan, which is applied in the field of preparation of nerve regeneration tubes, can solve the problems of high price and long-term administration of FK506, and achieve the effects of accelerating oriented growth and repair, avoiding long-term administration, and reducing costs

Inactive Publication Date: 2008-12-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the fact that FK506 currently used clinically is expensive and needs long-term administration, the present invention proposes a simple method to compound it into degradable chitosan to prepare drug-loaded chitosan for nerve regeneration with controllable degradation tube, to achieve sustained release of FK506 combined with degradation of chitosan to promote nerve regeneration and repair faster

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  • Method for prepraring medicine carrying chitosan neural regeneration tube with controlled degradation
  • Method for prepraring medicine carrying chitosan neural regeneration tube with controlled degradation
  • Method for prepraring medicine carrying chitosan neural regeneration tube with controlled degradation

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Embodiment 1

[0023] 1. Select chitosan with a molecular weight of 800kD and a degree of deacetylation of 93%, and prepare an aqueous acetic acid solution containing chitosan with a concentration of FK506 of 0.1% (w / v), in which the concentration of chitosan is 7% (w / v), The concentration of acetic acid is 2% (v / v), ultrasonic degassing;

[0024] 2. Spread the gel solution evenly on the outer surface of a stainless steel rod with a diameter of 2mm, immerse it in a 1mol / L sodium hydroxide solution, take it out after 5 minutes, wash a lot of water until the pH of the lotion is neutral, and take out the mold to obtain a white drug-carrying shell polymer. Sugar gel, such as figure 1 shown in a;

[0025] 3. Put the drug-loaded chitosan gel in the previous step on a stainless steel rod with a diameter of 1.8mm and dry it at 60°C for 30 minutes. Remove the mold and dry it naturally to obtain a drug-loaded chitosan nerve regeneration tube (such as figure 2 a);

[0026] 4. Application of drug-loaded c...

Embodiment 2

[0028] 1. Select chitosan with a molecular weight of 500kD and a degree of deacetylation of 80%, and prepare an aqueous acetic acid solution containing chitosan with a FK506 concentration of 1% (w / v), where the chitosan concentration is 4% (w / v), The concentration of acetic acid is 2% (v / v), ultrasonic degassing;

[0029] 2. Spread the gel solution evenly on the outer surface of a stainless steel rod with a diameter of 2mm, immerse it in a 0.1mol / L potassium hydroxide solution, take it out after 10 minutes, wash a lot of water until the pH of the lotion is neutral, take out the mold to obtain a translucent drug loading Chitosan gel (e.g. figure 1 b);

[0030] 3. Put the drug-loaded chitosan gel in the previous step on a stainless steel rod with a diameter of 1.8mm to dry naturally to obtain a drug-loaded chitosan nerve regeneration tube (such as figure 2 b);

[0031] 4. Application of drug-loaded chitosan nerve regeneration tube: 8 weeks after transplantation in Wister rats, the...

Embodiment 3

[0033] 1. Prepare an aqueous solution of acetic acid containing chitosan with a FK506 concentration of 4% (w / v), in which the chitosan concentration is 4% (w / v), the acetic acid concentration is 1% (v / v), and ultrasonic defoaming . The degree of deacetylation of chitosan is 70%, and the molecular weight is 200KD;

[0034] 2. Spread the gel solution evenly on the outer surface of a stainless steel rod with a diameter of 2mm, immerse it in a 2mol / L sodium hydroxide solution, take it out after 2 minutes, wash a large amount of water until the pH of the lotion is neutral, and take out the mold to obtain a transparent drug-carrying shell polymer. Sugar gel (e.g.figure 1 c);

[0035] 3. Put the drug-loaded chitosan gel in the previous step on a stainless steel rod with a diameter of 1.8mm and dry it at 40°C for 20 minutes. Remove the mold and dry it naturally to obtain a drug-loaded chitosan nerve regeneration tube (such as figure 2 c).

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Abstract

The invention relates to a method for preparing a controllable degradable drug loading chitosan nerve regenerative tube. The drug of FK506 which can promote growth of nerves is dispersed in acetic acid solution of chitosan for preparing stock solution, then is immerged into sodium hydroxide or potassium hydroxide solution in virtue of molds for freezing and forming, gelatin is bathed to lead the PH to be neutrality, the drug loading chitosan nerve regenerative tube is obtained by dryness. The animal experiment proves that, when the regenerative tube of the invention is used for bridging the deficient nerves, the slow released FK506 can improve the growth rate of the nerves, and the regenerative tube can be degraded in the growth process progressively, thus being beneficial to guided growth and repair of the damaged nerves.

Description

Technical field [0001] The invention relates to a method for preparing a nerve regeneration tube, in particular to a method for preparing a controllable degradable chitosan nerve regeneration tube containing a nerve growth-promoting drug FK506 (tacrolimus, trade name prograf) , Select chitosan with different degradation rates, load FK506 to prepare chitosan gel, and dry to obtain the drug-loaded chitosan nerve regeneration tube with controllable degradation. When used for nerve regeneration, FK506 is slowly released, and chitosan is gradually degraded, thereby quickly promoting the regeneration and functional repair of injured nerves. Background technique [0002] The repair of peripheral nerve injury has always been a problem of clinical concern. Although autologous nerve transplantation has a long history of application, there are still problems such as limited sources, difficulty in matching, and possible mismatch of nerves, which limit its application. With the development of...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/20A61F2/04
Inventor 马小军李晓霞王为张卫国于炜婷
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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