Method for extracting mushroom polysaccharide by using ultra-high pressure

A technology of lentinan and ultra-high pressure, which is applied in the field of extracting lentinan by using ultra-high pressure and extracting lentinan, to achieve the effect of shortening the extraction time

Inactive Publication Date: 2008-12-17
INFINITUS (CHINA) CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of ultra-high pressure extraction technology to extract lentinan has not been reported at home and abroad.

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0016] Concrete operation steps of the present invention are as follows:

[0017] a. Choose dried shiitake mushrooms

[0018] b. to crush

[0019] c. Add purified water

[0020] c.1 adding the crushed shiitake mushrooms into purified water, the mass ratio is 1:1-50;

[0021] d. Ultra-high pressure treatment

[0022] d.1 Pressure 100~600MPa

[0023] d.2 Temperature 20℃~80℃

[0024] d.3 Pressure holding time 1 ~ 10min

[0025] d.4 Repeat boosting and unloading for 1 to 3 times;

[0026] e. filter

[0027] f. Vacuum concentration

[0028] f.1 Temperature 40℃~70℃

[0029] f.2 Vacuum degree 85~98KPa

[0030] f.3 Time 30-90 minutes;

[0031] g. Ethanol precipitation

[0032] g.1 Add 2 to 5 times the volume of concentrated solution of 95% ethanol

[0033] g.2 Alcohol lake time 4 ~ 15h;

[0034] h. dry

[0035] h.140℃~70℃ drying;

[0036] i. Lentinan.

[0037] Now provide following embodiment, but the present invention is not limited to this embodiment:

[0038] a. C...

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Abstract

The invention discloses a method for extracting shiitake polysaccharides from shiitakes, which overcomes the defects that the current extracting method requires long time, has low efficiency and high energy consumption and damages active ingredients of the shiitake polysaccharides. The proposal adopted by the invention comprises the following steps of: crushing the selected dry shiitakes, adding extract, processing under super-high pressure, filtering, concentrating in vacuum, precipitating with alcohol, drying and finally obtaining shiitake polysaccharides. The high pressure is adopted by the invention to hold or relieve pressure of the shiitake extract, increase the differences between osmotic pressures in and out of cells and change the structure of cells, thereby easily dissolving out effective ingredients, keeping the physiologically active ingredients unchanged, killing a part of microorganisms by high-pressure processing and finishing extraction and sterilization in one step. Being a cold extracting technique, the super-high-pressure extracting method generates no high temperature during the whole extraction process, shortens the extracting time to a plurality of minutes and conserves the effective ingredients and has significant economic benefits and broad prospect of market development.

Description

technical field [0001] The invention relates to a method for extracting lentinan from shiitake mushrooms, in particular to a method for extracting lentinan by ultra-high pressure, and belongs to the field of medicinal material processing. Background technique [0002] my country is a big producer of edible fungi, among which the output of shiitake mushrooms ranks first in the world. Shiitake mushrooms are rich in protein, amino acids, fats, carbohydrates, vitamins and minerals and other nutrients. Among them, Lentinan in carbohydrates has many functions such as anti-virus, anti-tumor, regulating immune function and stimulating the formation of interferon. Lentinan is not only an ideal drug, but also an important functional factor of health food. It is the focus of common attention of the pharmaceutical and food industries today, and has broad development and application prospects. [0003] At present, the most commonly used extraction method of lentinan is water extraction,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/12A61P35/00A61P37/02A23L1/30A23L33/125
Inventor 杜冰杨公明温升南唐建李德灵
Owner INFINITUS (CHINA) CO LTD
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