Immunoregulation novel use of cinobufagin

A technology of immune regulation and cinobufagin, applied in the field of medicine

Inactive Publication Date: 2008-12-24
宋宇 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It has the effects of strengthening the heart, boosting blood pressure, local anesthesia, and anti-tumor, but its impact on the immune function of the body has not been reported

Method used

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  • Immunoregulation novel use of cinobufagin
  • Immunoregulation novel use of cinobufagin
  • Immunoregulation novel use of cinobufagin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] 1 Materials and methods

[0008] 1.1 Experimental animals

[0009] Healthy Balb / c mice, male ♂, 6-8 weeks old, weighing (20±2) g, were purchased from the Experimental Animal Center of the Basic Medical College of Jilin University, certificate number: SCXK (Ji 2003-0001).

[0010] 1.2 Drugs and reagents

[0011] 1.2.1 Source of Cinobufagin

[0012] Cinobufagin was purchased from China Institute for the Control of Biological Products.

[0013] 1.2.2 Reagents and preparation

[0014] Cinobufagin monomer is a fat-soluble drug, and DMSO (final volume-1 Penicillin, 100g·L -1 Streptomycin, 1% glutamine, 10% inactivated newborn calf serum, pH7.2), trypsin are all Gibco products, CD4 / L3T4-PE, CD8a / Lyt-2-FITC fluorescently labeled antibodies are all Southern Biotech product.

[0015] 1.3 MTT method to determine the transformation rate of mouse spleen lymphocytes

[0016] Spleen of mice was aseptically taken and prepared into 5×10 6 pcs L -1 100 μL per well of lymphocyte ...

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Abstract

Through a method of MTT, the individual effects of cinobufagin and the influence of combined effects of the cinobufagin and canavaline A or lipopolysaccharide on the reproduction or conversion of splenic lymphocytes of a normal mouse are detected; through the method of MTT and neutral red phagocytosis experiment, the influence of CBG on the energy metabolism level and the phagocytosis function of macrophages in the abdominal cavity is detected; through flow cytometry, the influence of the CBG on the splenic lymphocytes period of the normal mouse and the expression of the surface antigens CD4 and CD8 of the T lymphocyte in the spleen is detected. The invention validates that the cinobufagin has the effect of enhancing the immunity.

Description

1. Technical field [0001] The invention relates to the immunoregulatory effect of cinobufagin, belonging to the field of medicine. 2. Background technology [0002] Cinobufagin (CBG) is one of the active ingredients of toads, and its chemical name is: 5beta-Bufa-20, 22-dienolide, [0003] 14, 15beta-epoxy-3beta, 16beta-dihydroxy-, 16-acetate (8CI). Its structure is as follows: [0004] [0005] It has the effects of strengthening the heart, boosting blood pressure, local anesthesia, and anti-tumor, but its impact on the immune function of the body has not been reported. The invention studies the regulating effect of cinobufagin on the immune function of mice, and preliminarily clarifies the mechanism of its immune enhancing effect, so as to provide a certain scientific basis for finding new and effective traditional Chinese medicine immune enhancing agents. 3. Contents of the invention [0006] The effect of CBG on the proliferation and transformation of spleen lymph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P37/02
Inventor 宋宇邓旭明王大成
Owner 宋宇
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