Method for rapidly testing cysteine in water solution
A cysteine and aqueous solution technology, applied in the field of cysteine detection, can solve the problems of inability to be fully automated, complicated in operation, expensive and the like, and achieve the effects of low production cost, simple detection process and low price
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Embodiment 1
[0050] Embodiment 1: The method for rapid detection of cysteine in aqueous solution by spectrometry
[0051] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and 2 x 10 -3 Cysteine aqueous solution of M; add 2mL of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 25 μl of xylenol orange solution of M was added to the cuvette, and the solution turned from colorless to yellow at this time, detected on a UV-visible spectrophotometer, and there was a maximum absorption at 441nm; in the above-mentioned cuvette, add 2 ×10 -3 25 μ l of copper nitrate solution of M, at this moment, the solution was changed from yellow to purple, and detected on the UV-visible spectrophotometer, it was found that the maximum absorption peak changed from the above-mentioned 441nm to 572nm, and the UV-visible absorption spectrum was shown in figure 1 Take cysteine aque...
Embodiment 2
[0052] Embodiment 2: the method for the rapid detection of cysteine in aqueous solution by solution colorimetry (kit method)
[0053] Kit preparation:
[0054] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and 2 x 10 -3 M cysteine aqueous solution; take five colorimetric tubes, respectively add 2ml of HEPES buffer solution, 25μl 2×10 -3 The xylenol orange solution of M, at this time the solution turns from colorless to yellow, denoted as R 1 , R 2 , R 3 , R 4 , R 5 ; in R 2 , R 3 , R 4 , R 5 Add 2×10 -3 M copper nitrate solution 25μl, at this time the solution changed from yellow to purple, use a micro-sampler to absorb 35μl, 65μl, 100μl cysteine and add to R 3 , R 4 , R 5 In, colorimetric visual view image 3 , R 3 , R 4 , R 5 The represented cysteine concentrations are 35 μM, 65 μM, 100 μM, respectively.
[0055] Unknown concentration cys...
Embodiment 3
[0057] Embodiment 3: the method for rapidly detecting cysteine in aqueous solution by test paper colorimetry
[0058] Prepare HEPES (10mM) buffered aqueous solution with pH 6.0, and prepare 2×10 -3 M copper nitrate aqueous solution, 2×10 -3 M xylenol orange aqueous solution and cysteine aqueous solution with concentrations of 0.01, 0.04, 0.08, and 0.1 mM; 25 μl of Cu 2+ Solution and 25 μl of xylenol orange solution were added to 2ml of HEPES (10mM) buffer solution. After stirring evenly, the solution was purple-red. Immerse a large piece of filter paper in the purple-red solution for 10 seconds, take it out and dry it in the air, and then Repeated infiltration and drying for 3 times, the cysteine test paper can be used, and the test paper is cut into strips for use;
[0059] Take 5 strips of strip test paper, soak them in the cysteine aqueous solution with a concentration of 0, 0.01, 0.04, 0.08, and 0.1mM for 3 seconds, and take them out to obtain purple (blank), pur...
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