Technique of preserving bailing mushroom using chitosan to coat film
A technology of Bailing mushroom and chitosan solution is applied in the preservation of Bailing mushroom, and the field of preservation of Bailing mushroom is treated with chitosan coating film.
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Embodiment 1
[0022] The configuration of embodiment 1 preservation liquid
[0023] Weigh 25g, 50g and 75g food-grade chitosan respectively, dissolve in 5L 1% acetic acid solution in hot water at 60°C respectively, and stir with a glass rod while heating until all the chitosan is dissolved. Finally, the pH value was adjusted to 6.0 with NaOH, that is, 0.5%, 1.0% and 1.5% chitosan coating fresh-keeping solutions were configured.
[0024] Weigh 25g CaCl 2 In 5L distilled water, make up 0.5% CaCl 2 solution.
Embodiment 2
[0025] Embodiment 2 carries out fresh-keeping treatment to Bailing mushroom
[0026] Soak the picked fresh Bailing mushroom fruit bodies in the prepared fresh-keeping solutions respectively, soak the Bailing mushrooms in 0.5%, 1.0% and 1.5% chitosan-coated fresh-keeping solutions for 30s, and soak them in 0.5% CaCl 2 Soak Bailing mushroom in the solution for 5 minutes. After soaking, drain all the Bailing mushrooms overnight to make a film. Then every 2 are packed in a plastic tray and packed into a box with plastic wrap. The unsoaked Bailing mushroom was used as the control (CK). After packing, put all Bailing mushrooms into a 4°C cold storage. Samples were taken every 4 days, and 6 fruiting bodies (3 boxes) were randomly taken from each treatment group each time.
Embodiment 3
[0027] Embodiment 3 preservation result and analysis
[0028] 3.1 The measured items are:
[0029] Weight loss rate; superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO) and protease activities; malondialdehyde (MDA) content; soluble protein content; total sugar and reducing sugar content.
[0030] 3.2 The measurement method is:
[0031] 3.2.1 Sensory evaluation
[0032] Five fixed persons were used to evaluate the sensory quality of Bailing mushroom, and the evaluation items included browning, softening, odor, aerial hyphae, and cracking of mushroom surface.
[0033] 3.2.2 Enzyme Extraction
[0034] Take 2g of the fruiting body of Bailing mushroom by quartering, add 10ml of phosphate buffer (0.05M, pH 7.0) and a small amount of quartz sand in a mortar, grind in an ice bath until a uniform paste, and then centrifuge for 10min (15000g, 4°C ). The obtained supernatant is the crude enzyme solution.
[0035] 3.2.3 Determination of SOD
[0036] The determinat...
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