Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell

Abstract

The invention belongs to the aquiculture field, in particular to an eutelegenesis method for improving the ovum fertilization rate of spotted halibut. The method is an eutelegenesis technology for sea fish spotted halibut ripe fish, which solves the problems that because the ovulatory cycle of the spotted halibut which is artificially raised is unstable, the inside of ovarian cavity is usually overripe, and among the artificially extruded ovum, the overripe ovum is often mixed with normal ovum, the fertilization rate of eutelegenesis is very low, and batch fertilized eggs are difficult to be obtained, etc. In the eutelegenesis method for improving the ovum fertilization rate of spotted halibut, the spotted halibut ovum is fertilized in high-salt seawater after being separated in the high-salt seawater. The method improves the fertilization rate of the spotted halibut ovum, obtains batch fertilized eggs, lays technical foundation for adding a novel aquaculture breed for the marine aquaculture industry of our country, and has significant meaning in developing the industrialization of spotted halibut.

Description

technical field [0001] The invention belongs to the field of aquaculture, and specifically relates to a method for artificially inseminating eggs of spotted flounder through high-salt sea water to improve the fertilization rate. Background technique [0002] The spotted flounder is mainly distributed in the Sea of ​​Okhotsk, the Northwest Seas of Japan, the East and Southwest Seas of the Korean Peninsula, and the Yellow and Bohai Seas of China. It is a cold-temperate bottom fish in the Northwest Pacific Ocean. The spotted star flounder is larger in size, faster in growth, less in bony spurs, smaller in viscera, and more edible. It is an excellent breeding species. [0003] The ovulation cycle of spotted flounder is unstable under artificial rearing, and the ovarian cavity is often overripe. Artificially extruded eggs are often mixed with overripe eggs and normal eggs. The fertilization rate of artificial insemination is very low, and it is difficult to obtain batches of fer...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide an artificial insemination method for improving the fertilization rate of spotted star flounder eggs, which is an artificial insemination technique for marine fish spotted star flounder broodstock, and solves the problem of irregular ovulation cycles of spotted star flounder due to artificial breeding. Stable, the ovarian cavity is often overripe, artificially extruded eggs are often mixed with overripe eggs and normal eggs, the fertilization rate of artificial insemination is very low, and it is difficult to obtain batches of fertilized eggs, etc.