Method for simultaneously analyzing twenty amino acids in tobacco and product thereof

A technology for amino acids and tobacco, applied in the field of chemical analysis and detection, to achieve high sensitivity, high sensitivity and selectivity, and simplify sample pretreatment

Active Publication Date: 2009-01-28
CHINA TOBACCO GUANGDONG IND
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no relevant technical reports on the simultaneous rapid analysis of 20 underivatized amino acids in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously analyzing twenty amino acids in tobacco and product thereof
  • Method for simultaneously analyzing twenty amino acids in tobacco and product thereof
  • Method for simultaneously analyzing twenty amino acids in tobacco and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Instruments, materials and reagents

[0034] LC-MS LTQ Liquid Chromatography-Ion Trap Mass Spectrometer from Thermo Electric Company, equipped with autosampler, quaternary gradient mass spectrometer pump, LTQ ion trap mass spectrometer charged spray ionization source (ESI), Xcalibur 1.4SR1 system control software; Italy Claind N2LCMS Nitrogen generator; French Millipore Element A10 pure water machine; American Cole-Parmer 8892 ultrasonic cleaner; Sartorious CP225D electronic balance (Max 220g, d=0.01mg (80g), 0.1mg (220g)); Whatman 0.2μm nylon needle type filter; tobacco powder.

[0035] Mixed amino acid standard solution (Fluka Company), containing 17 kinds of amino acids, the concentration is 1mM each; internal standard L-norvaline (Shanghai Livzon Dongfeng Biotechnology Co., Ltd.); asparagine water (Shanghai Boao Biotechnology Co., Ltd. Co., Ltd.); tryptophan, proline and glutamine (Sinopharm Chemical Reagent Co., Ltd.); γ-aminobutyric acid (Sigma); L-piperidine-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method used for simultaneously analyzing 20 types of amino acids in tobaccos and tobacco products, adopts the coupling of liquid chromatogram and electrospray tandem mass spectrum, and comprises the following experimental steps: a tobacco sample is preprocessed, extracting solution is obtained and eluted with binary flow phase gradient on a reversed phase chromatography column, two standard curves are produced, and liquid chromatogram-electrospray ion trap tandem mass spectrometry is adopted for analyzing and detecting the 20 types of amino acids in the tobaccos under a positive ion mode. The sampling of the tobacco sample is directly implemented after extraction and filtration and other preprocessing steps including derivation and solid phase extraction are avoided in the method of the invention, thus providing simplicity, convenience and fastness; the method has lowered chromatogram separable degree requirement of detected objects, can effectively and simultaneously solve the co-flow problem and the separated detection problem of amino acid isomers, builds a quickly simultaneous analyzing method of the 20 types of non-derivatized amino acids in the tobaccos, has good reproducibility, high sensitivity and selectivity, greatly simplifies analyzing steps, raises analyzing efficiency, and can be successfully applied into the analytic determination of the amino acids in various tobacco samples.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for simultaneously analyzing 20 kinds of amino acids in tobacco and its products. Background technique [0002] Amino acids are an important part of tobacco leaves, and are tobacco components closely related to smoke aroma, cigarette quality and brand characteristics. Amino acids participate in the non-enzymatic brown reaction in tobacco leaves, namely the Maillard reaction, which can produce a variety of important Aroma ingredients. Therefore, the determination of amino acids in tobacco is of great significance to formula development, process production and quality control. [0003] Because there are many kinds of amino acids in tobacco, and they have three different chemical properties: acidic, neutral and basic, and the matrix of tobacco is complex, it is difficult to simultaneously measure multiple amino acids in tobacco. Currently, liquid c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/89
Inventor 黄翼飞
Owner CHINA TOBACCO GUANGDONG IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap