Zingiber plant extract

An extract, the technology of Zingiberaceae, applied in the field of Zingiber officinale plant extracts, can solve the problems of high price and limited drug production capacity

Inactive Publication Date: 2009-02-11
NATBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this inhibitor is limited by many factors, such as the limited production capacity of this drug, and it is too expensive for many countries

Method used

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  • Zingiber plant extract
  • Zingiber plant extract
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1 Ginger protease inhibits influenza virus

[0084] Cell membrane proteins of viruses such as sialidase and hemagglutinin of influenza virus are proline-rich and have multiple zingiberinase hydrolysis sites. These proteins are essential for the infection process. Its cleavage can inhibit virus infection, proliferation and remove superantigens, antigens of hemagglutinin protein and damaging high-level cytokines released by related T cells.

[0085] Sialidases, including the N1 variant found in H5N1 avian influenza, have multiple potential ginger protease hydrolysis sites adjacent to pro-3, 48, 120, 154, 167, 169, 198, 167, 169, 198, 246, 272, 283, 302, 326, 328, 340, 377, 410, 420, 431, 458, as shown below. These include potential sites on the protein stem-loop adjacent to amino acids that stabilize the binding of the aforementioned inhibitors and host cell sialic acid to the enzyme.

[0086] Sialidase N1

[0087] 1 mnpnqkitti gsicmvigiv slmlqignii siwvshs...

Embodiment 2

[0159] Example 2 Zingiberin inhibits Ross River Virus.

[0160] The antiviral drug assay used to detect the inhibitory activity of the drug against the virus was also used to detect 10 -5 and 10 -6 Effect of Zingiberin on Dilutions of Mosquito-Transmitted Virus, Ross River Virus (RRV). The virus was mixed with 7.5 units activity / mL of ginger protease and incubated at pH 7.2 for 2 hours. This was then added to a confluent monolayer of "Vero" cells. Count the plaques produced by the virus.

[0161] Table 2

[0162] Plaque Assay of Ross River Virus Incubated with Zingiberin (7.5 Units / mL) in Vero Cells

[0163] RRV

[0164] Zingiberin concentration in this type of assay depends on the cells adhered to the surface of the slide, because Zingiberin will cause cells such as "Vello" cells to aggregate from the surface of the slide, so in this type of assay Higher concentrations of ginger protease cannot be used in . At 7.5 unit activity / mL, ginger protease can inhibit...

Embodiment 3

[0165] Example 3 Ginger protease inhibits Murray valley encephalitis (Murray Valley Encephalitis)

[0166] The ability of 330 units / mL ginger protease to inhibit the replication of Murray valley encephalitis in serum-free medium was studied in "Vello" cells pre-infected with a certain dilution range of virus at 37°C for one hour. The virus dilution that can detect the virus in 50% of the wells is 10 in the absence of ginger protease -6 , it is 10 at 330 units / mL ginger protease -5.3 . This equates to approximately 5-fold inhibition of viral replication.

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Abstract

The present invention relates generally to plant extracts and / or components isolated therefrom which exhibit desirable properties in relation to therapy. More particularly, the present invention relates to extracts and components isolated thereof from the plant genus Zingiber and in particular from the rhizome of the species Zingiber officinale (also known as ginger) which comprise activities having broad applicability in the field of inhibition and treatment of infections by pathogenic micro-organisms including viruses, bacteria, protozoa and parasites.

Description

technical field [0001] The present invention relates to plant extracts and / or components isolated from plant extracts having desirable therapeutically relevant properties. In particular, the present invention relates to rhizome extracts of the genus Zingiber, especially the species Zingiber officinale (also known as ginger), and components isolated from the extracts, which contain activities broadly applicable to Inhibition and treatment of pathogenic microbial infections including viruses, bacteria, protozoa and parasites. Background technique [0002] Bibliographic details of publications cited by authors in this specification are listed centrally at the end of this note. [0003] Reference to any prior art in this specification is not and should not be considered as an acknowledgment or any form of suggestion that such prior art forms part of the common general knowledge in any country. [0004] Tissue extracts of monocotyledonous and dicotyledonous plants provide a lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9068A61K38/48A61P31/00A61P33/00C12N9/50A61K125/00
CPCC12N9/50A61K36/9068A61P31/00A61P31/16A61P33/00C12N9/63A61K2300/00
Inventor C·J·霍金斯
Owner NATBIO
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