Sulfating numerator modification method of epimedium brevicornum polysaccharide

A technology of epimedium polysaccharide and modification method, which is applied in the field of sulfated molecular modification, can solve the problem of low biological activity of polysaccharides, achieve good antiviral effect, improve the effect of enhancing immunity, and enhance the effect of enhancing immune effect

Inactive Publication Date: 2009-02-18
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Technical Problems The present invention aims at the problem of low biological activity of polysaccharides in traditional Chinese medicines, and provides a method for sulphating molecular modification of polysaccharides from Epimedium to achieve the purpose of further improving the biological activity of polysaccharides from Epimedium

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  • Sulfating numerator modification method of epimedium brevicornum polysaccharide
  • Sulfating numerator modification method of epimedium brevicornum polysaccharide
  • Sulfating numerator modification method of epimedium brevicornum polysaccharide

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Embodiment Construction

[0017] 1. Extraction and Purification of Epimedium Polysaccharides

[0018] Take 2kg of epimedium and add 8 times of water to boil, keep the fire low for 1h, decoct twice, combine the filtrate and concentrate to 2000mL; slowly add ethanol to 70% at room temperature, stir well, let it stand for 24h to settle; take the precipitate to evaporate ethanol, add Redissolve in 2000 mL of distilled water and let it stand for 24 hours; take the supernatant and add ethanol to 70%, stir well, and let it stand for 24 hours; take the precipitate, evaporate the ethanol, and freeze-dry to obtain crude epimedium polysaccharide.

[0019] 2. Purification of Epimedium Polysaccharides

[0020] (1) Trichloroacetic acid method to remove protein Epimedium polysaccharide is made into 10% solution with distilled water, slowly adds 10% trichloroacetic acid solution, shakes while adding, until trichloroacetic acid accounts for 0.3% of total volume, fully After shaking well, let it stand for 4 hours, cent...

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Abstract

The invention discloses a sulfating modification method of epimedium polysaccharide, belonging to the technical field of structural improvement. The method comprises the steps of extracting and purifying the epimedium polysaccharide, using chlorosulfonic acid-pyridine method for sulfating molecular modification, setting 9 modification conditions according to L9(3<4>) orthogonal test based on 3 factors of the reaction temperature, the reagent proportion and the reaction time and respectively modifying to obtain 9 epimedium polysaccharides. The optimum modification conditions are determined that the reaction temperature is 80 DEG C, the proportion of chlorosulfonic acid and pyridine is 1:8 and the reaction time is 1h through systematically comparing the immune enhancing activity and the antiviral activity of the 9 sulfated epimedium polysaccharides. The obtained sulfated epimedium polysaccharide has the highest sugar content and the strongest activity. The sulfating modification method of epimedium polysaccharide can obviously enhance the immune enhancing activity and the antiviral activity of the epimedium polysaccharide.

Description

1. Technical field [0001] The invention discloses a sulfated molecular modification method of epimedium polysaccharides, which belongs to the technical field of structure modification of polysaccharides of traditional Chinese medicines. 2. Background technology [0002] Polysaccharides are another important biological macromolecular substance in addition to proteins and nucleic acids in organisms. They have many functions such as resisting infection, enhancing immunity, preventing and treating tumors, viral hepatitis, rheumatoid disease, AIDS and other immune damage or immunodeficiency diseases, and anti-oxidation. aspects of function and biological activity. With the rapid development of chemistry and biology and the improvement of separation technology, the biological functions of polysaccharides, especially as living substances to participate in the full time and space functions of life, have broken through the traditional concept of polysaccharides as supporting tissues ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P31/12
Inventor 胡元亮卢宇王德云张宝康刘家国
Owner NANJING AGRICULTURAL UNIVERSITY
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