Rice flavoprotein gene and use

A protein and rice technology, applied in application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2012-01-04
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on whether this gene exists in crops, and the functional research of this gene in crops

Method used

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  • Rice flavoprotein gene and use
  • Rice flavoprotein gene and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1 Rice Flavoprotein Gene

[0126] It has been identified that the full-length ORF of the rice flavoprotein gene OsHAL3 is 663 nucleotides, encoding 220 amino acids, and its amino acid sequence is shown in SEQ ID NO: 2 or see figure 1 B; its nucleotide sequence is shown in SEQID NO: 1 or see figure 1 a.

Embodiment 2

[0127] Example 2 Effect of OsHAL3 gene on rice salt tolerance

[0128] The OsHAL3 gene was placed under the control of the CaMV35S promoter in the positive direction and constructed on the rice expression vector pHB; then, it was introduced into the rice variety "Zhonghua 11" by the Agrobacterium-mediated method.

[0129] Salt stress analysis of the positively transformed plants showed that the overexpression of the OsHAL3 gene led to a significant increase in the salt tolerance of the transgenic rice lines, which was due to the Na + ions drop significantly while the K + caused by a significant increase in ions, see figure 2 .

[0130] Depend on figure 2 A and figure 2 B shows that after 10 days of culture with 120mM NaCl, the growth of transgenic rice overexpressing OsHAL3 is basically normal, while the growth of transgenic rice with pHB empty plasmid is poor, and the leaves wither are more serious; after 25 days of cultivation, the transgenic rice overexpressing OsHAL...

Embodiment 3

[0132] Example 3 OsHAL3 gene improves rice seed storage tolerance

[0133] Although the seeds of the overexpressed OsHAL3 gene transgenic line were stored for 2 years, the seed germination rate was as high as 95%, while the germination rate of the transgenic rice or wild-type control seeds with the pHB empty plasmid was only 25%, see image 3 a.

[0134] This clearly shows that the OsHAL3 gene plays an important role in the storage tolerance of rice seeds, and has important application prospects.

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Abstract

The invention belongs to the gene technical field, and discloses a paddy rice flavoprotein gene and application thereof. The paddy rice flavoprotein gene can be used for regulating the crop growth, improving crop resistance to salt stress, improving storage resistance of crop seed, prolonging the life of the crop seed, and improving the germination rate of the seed. The inventor further finds outthat the activity and the expression of the paddy rice flavoprotein gene are regulated by light so as to regulate the growth of the paddy rice. The paddy rice flavoprotein gene has wide application in crop salt tolerance, crop growth regulation and crop storage resistance and breeding.

Description

technical field [0001] The invention belongs to the field of gene technology, more specifically, the invention relates to a rice flavin protein gene and its application. Background technique [0002] The flavoprotein gene HAL3 was first cloned and discovered in yeast, and it controls the cell division and ion balance of yeast. Plant Arabidopsis thaliana also found flavoprotein gene AtHAL3(a, b), overexpression of AtHAL3a in Arabidopsis can improve Arabidopsis Na + and osmotic stress resistance, accelerated the growth of Arabidopsis, while the growth of antisense Arabidopsis lines was slower than that of the wild type, and the plants were dwarf. [0003] Comparing the amino acid sequences of AtHAL3a and ScHAL3 (yeast), it is found that they have a relatively conserved FMN (flavin mononucleotide) binding site, that is, the binding site of the trimer, which implies that ScHAL3 and AtHAL3a have similar trimer structure and function. The results of crystal structure analysis s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82
Inventor 林鸿宣孙世勇晁代印施敏高继平朱美珍
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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