Preparation of novel tuberculosis vaccine and use thereof

Abstract

The invention relates to a recombinant virus carrier which can co-express tubercle bacillus Ag85a, ESAT6 and / or Ag85a / ESAT6 fusion protein as well as any immune auxiliary factor; an expression cassette containing at least one type of tubercle bacillus Ag85a coding sequence, ESAT6 coding sequence and Ag85a / ESAT6 fusion protein coding sequence and the any immune auxiliary factor coding sequence is inserted into a gene group DNA of the recombinant virus carrier. The invention also relates to a host cell containing the virus carrier, a pharmaceutical composition and a preparation method thereof. The recombinant virus carrier can co-express a plurality of antigens of m.tuberculosis and / or cell factors, and has improved prevention and / or curing effect to tuberculosis.

Description

technical field

[0001] The invention relates to the fields of bioengineering and immunization, in particular to a novel tuberculosis vaccine and a preparation method thereof. Background technique

[0002] In the past 20 years, tuberculosis, which was once controlled at a very low level, is showing a resurgent trend worldwide. There are currently 20 million tuberculosis patients in the world, 95% of whom are in developing countries. If not controlled, 300 million people will be infected by Mycobacterium tuberculosis in the next 10 years. To this end, the World Health Organization declared a global tuberculosis emergency in 1993, and reiterated its action to curb tuberculosis in 1998.

[0003] The causative bacterium of tuberculosis is Mycobacterium tuberculosis, which belongs to the Actinomycetes Mycobacteriaceae Mycobacterium genus. Mycobacterium tuberculosis is mainly transmitted through the respiratory tract, and can also enter the human body through the digestive tract....

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