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Use of polysaccharide sulfate as anti influenza virus medicine

一种抗流感病毒、流感病毒的技术，应用在抗病毒剂、医药配方、含有效成分的医用配制品等方向

Inactive Publication Date: 2009-03-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In the reported literature and patents, the average molecular weight of natural marine red algae polysaccharides (including carrageenan and Porphyra laver polysaccharides) is chemically modified and processed. The individual or synergistic inhibitory effects of red algal polysaccharide sulfate (carrageenan polysaccharide sulfate, laver polysaccharide sulfate) and α-D-(1→6)-glucan sulfate on influenza A and B viruses, no see the report

Method used

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  • Use of polysaccharide sulfate as anti influenza virus medicine
  • Use of polysaccharide sulfate as anti influenza virus medicine
  • Use of polysaccharide sulfate as anti influenza virus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of Eucheuma polysaccharide (carrageenan)

[0026] Weigh a certain amount of dry Eucheuma auris (purchased from Xiamen Fisheries Research Institute), cut into pieces and wash, add 50 times the weight of hot water, stir and heat at 95°C for 3 hours. The crude polysaccharide extract was centrifuged at 5000 rpm for 10 minutes, and the supernatant was collected. Add KCl to make the final concentration 5%, stir evenly, let stand overnight, and centrifuge at 5000 rpm for 10 minutes to separate insoluble matter. Dissolve the insoluble matter with distilled water, then add ethanol at a ratio of 3:1 (v / v), let it settle for 60 minutes, dry it under vacuum at 60°C, and grind it into a fine powder to obtain refined carrageenan powder. The molecular weight of the prepared carrageenan is higher than 500,000, and the substitution degree of sulfate group per monosaccharide is 0.45.

Embodiment 2

[0027] Embodiment 2: the preparation of laver polysaccharide

[0028] Weigh a certain amount of dry laver (produced in Pingtan, Fujian), cut it into pieces and wash it, add 50 times of distilled water to soak overnight, mash the laver with a masher, freeze it at -20°C, take it out and thaw it in microwave. After repeating 3 times, stir and extract in a 60°C water bath for 30 minutes, centrifuge at 3000rpm for 20 minutes, put the supernatant separately, add distilled water to the laver residue and repeat the extraction 2 times, combine the 3 supernatants, concentrate under reduced pressure at 50°C, and freeze-dry. get crude polysaccharides.

[0029] The laver crude polysaccharide was formulated into a 2% solution, and sulfuric acid was added to adjust the pH to 2.0. The polysaccharide solution flows through the regenerated 732 cation exchange resin column, which can effectively remove protein. The column solution was collected, concentrated to 3% w / v, and then the polysacchar...

Embodiment 3

[0030] Embodiment 3: the preparation of specific molecular weight carrageenan polysaccharide

[0031] The carrageenan (or commercial carrageenan) prepared in Example 1 was formulated into a 5% glue solution, placed in a constant temperature water bath, and fully mixed. Add sulfuric acid to adjust the pH to 2.5, and react at a constant temperature of 90°C for 120 minutes. After the reaction, neutralize with 1N NaOH solution immediately, and then filter with ultrafiltration membranes with different molecular weight cut-offs to prepare carrageenan polysaccharides with different molecular weights. The prepared carrageenan polysaccharide with specific molecular weight is spray-dried and used for the preparation of carrageenan polysaccharide sulfate.

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Abstract

The invention relates to an application of alpha-D-(1-6)-dextran sulfate with the average molecular weight range of 1000-100000 and the degree of substitution of monosaccharide sulfate of 0.2-3.0 as the preparation of anti-influenza virus drugs. The invention prepares a practical drug formulation of the alpha-D-(1-6)-dextran sulfate with the average molecular weight range of 1000-100000 and the degree of substitution of monosaccharide sulfate of 0.2-3.0 and determines that the polysaccharide sulfate has the use of synergistic anti-A and B-type influenza virus independently or after composing a compound with amantadine, ribavirin and other prior anti-influenza virus drugs.

Description

technical field [0001] The invention belongs to the field of synthetic pharmacy, and relates to the application of polysaccharide sulfate as preparation of anti-influenza virus drugs, in particular to marine red algal polysaccharide sulfate and α-D-(1→6)-dextran sulfate as preparation of anti-influenza virus drug application. technical background [0002] Influenza is an acute viral respiratory infectious disease caused by influenza virus that seriously endangers human health, and is known as one of the viral infectious diseases with the highest fatality rate. There have been several large-scale epidemics of influenza in history, which brought serious disasters to mankind. Although a variety of therapeutic drugs have been developed, the harm of influenza and the losses it causes cannot be ignored. influenza. my country is a place where influenza frequently occurs, and there are local outbreaks basically every year. For this reason, my country's Ministry of Health has lis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737A61P31/16
Inventor 郭养浩石贤爱孟春
Owner FUZHOU UNIV
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