Method for detecting cyanolabe in textiles by reversed phase high performance liquid chromatography

Abstract

The invention discloses a method for detecting cyanine in textile through reversed-phase high-efficiency liquid chromatography. The method comprises the following steps: (1) a solution of a sample to be detected is prepared; (2) a standard solution is prepared; (3) the standard solution is taken out, is injected to a high-efficiency liquid chromatographic instrument and is mensurated through a reversed-phase high-efficiency liquid chromatography method; the peak-out position of the cyanine is mensurated by a liquid chromatography-mass spectrometry instrument; the peak-out area is recorded; with concentration as an abscissa and the peak-out area as an ordinate, a standard curve equation is manufactured; and (4) according to the method in step (3), the cyanine and the peak-out area in the solution of the sample to be detected are mensurated; and the obtained standard curve is used to obtain the content of the cyanine in the sample to be detected through calculation. The method can accurately, reliably and sensitively mensurate the cyanine and the content of the cyanine in the textile.

Description

technical field [0001] The invention relates to a method for detecting blue pigment in textiles, in particular to a method for detecting blue pigment in textiles by reverse-phase high performance liquid chromatography. Background technique [0002] At present, about two-thirds of the synthetic dyes in the world market are made on the basis of azo chemistry, that is, about two-thirds of the synthetic dyes are azo dyes, and it is estimated that there are about 2,000 varieties with an annual output of nearly 600,000 tons. According to the application category of dyes, they belong to acid dyes, ice dyes, direct dyes, reactive dyes, disperse dyes, cationic dyes, basic dyes and solvent dyes. [0003] 1 Importance and harm of azo dyes [0004] The two basic reactions in the manufacture of azo dyes are diazotization and coupling. The reaction of aromatic primary amine (also known as diazonium component) and nitrous acid to form diazonium salt is called diazotization reaction. Fact...

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Problems solved by technology

[0006] ② Due to the use of carcinogenic aromatic amines as raw materials, and the conversion rate of most coupling reactions is not quantitative, these carcinogenic aromatic amines are entrained in the final product;

[0038] (3) The conditions of extraction and concentration are different in applicability to carcinogenic aromatic amines, especially aromatic amines that are easily oxidized;

[0040] (5) The precision and repeatability of the method are poor

[0042] 3 Chemical composition and hazards of blue pigment

[0046] And because the azo dye monitoring method is prohibited in German textiles by reducing the azo dye, measure the reduction product to determine the content of the azo dye, use this method to measure the blue pigment, the substance in the solution after extraction is very complicated (see figure 1 ), under the premise of unknown blue pigment structural formula, it is difficult to judge which peaks in the chromatogram are related to blue pigment, and it is impossible to accurately and reliably detect blue pigment and its content in textiles

And at present, our country has not publicly released a feasible method for detecting blue pigment, so that the inspection work of this project cannot be carried out. Therefore, it is urgent to study an accurate, reliable, sensitive inspection method and related technologies to meet the needs of the development of green textiles.

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