Method for detecting chlorpromazine by colloidal gold immune chromatography test

An immunochromatography, colloidal gold technology, applied in the field of bioengineering, to achieve the effect of strong pertinence, single detection object, and high accuracy
CN101424688AInactive Publication Date: 2009-05-06SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2009-05-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method using a colloidal gold immuno chromatographic assay to test chlorpromazine in the technical field of biological engineering. CPZ hapten is connected with aminobenzoic acid through diazo reaction, and the CPZ hapten and KLH and BSA amidogens form peptide linkages by carboxyl on benzene rings so as to form the CPZ conjugates of CPZ-KLH and CPZ-BSA. The antigen CPZ-KLH is used for preparing polyclonal antibodies, the antigen CPZ-BSA is used for an indirect Elisa adsorption assay for testing polyclonal antibody titers, the polyclonal antibodies are labeled by the colloidal gold used as a tracer label, the CPZ-BSA antigen is immobilized on a test line on a membrane, and anti-rabbit polyclonal antibodies are immobilized on a control line; and the gold-labeled antibody, the corresponding antigens and the antibodies are specifically bound to perform the operation of colour development through isolation, and negative and positive results are displayed by the colour development of the test and the control lines.The invention can directly test chlorpromazine micromolecules in samples and has the advantages of fast test speed and short required time.
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Description

technical field

[0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for detecting chlorpromazine by colloidal gold immunochromatography test. Background technique

[0002] Chlorpromazine (CPZ, N, N-dimethyl-2-chloro-10H-phenothiazine-10-propylamine hydrochloride) is a good sedative, which has a good inhibitory effect on the central nervous system. Appropriate addition in animal feed can significantly increase daily gain, reduce feed consumption, and can be used as an anti-stress drug to prevent stress syndrome and reduce mortality during transportation. CPZ is mainly metabolized in the liver, and most of it is excreted with urine, and the excretion method is slow, which is easy to produce drug residues. CPZ has an inhibitory effect on the nervous system, respiratory system and circulatory system of the human body, and can enter the fetal blood circulation through the placental barrier. People with mild pois...

Claims

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