Culture medium for human peripheral blood source hemopoietic stem cell

Abstract

The invention relates to a culture medium for human peripheral blood hematopoietic stem cells, and particularly provides a culture medium for performing short-term culture on the human peripheral blood hematopoietic stem cells acquired in vitro to improve the antitumor activity of the human peripheral blood hematopoietic stem cells. The culture medium capable of improving the killing capability of the human peripheral blood hematopoietic stem cells to the tumor is characterized in that the culture medium comprises the following concrete compositions: 1000ml of Dulbecco improved basic culture medium (IMDM) containing Iscobe, 0.1 to 10mu g / ml fatty acid, 1.5mu g / ml cholesterin, 5 to 20mu g / ml glyceride, and 300mu g / ml transferring , wherein the buffer solution is sodium bicarbonate or HEPES, and the pH value of the culture medium is 7.0.

Description

【Technical field】 [0001] The present invention relates to a culture medium for human peripheral blood-derived hematopoietic stem cells. Specifically, the invention provides a medium for short-term culture of human peripheral blood-derived hematopoietic stem cells collected in vitro to improve their anti-tumor activity. The invention also relates to a medium for short-term culture of hematopoietic stem cells. Finally, the present invention also relates to a transplantation method of hematopoietic stem cells, which can significantly improve the viability of patients. 【Background technique】 [0002] Hematopoietic stem cells (HSCs) are a small group of hematopoietic precursor cells with high self-replication ability and multilineage differentiation potential. Its basic features are: 1) High self-renewal or self-replication ability; 2) Differentiation and generation of all types of blood cells. Under normal circumstances, hematopoietic stem cells continuously produce a large nu...

AI Technical Summary

Problems solved by technology

[0011] 1) In the prior art, there is no short-term in vitro culture method for improving the anti-tumor activity of hematopoietic stem cells, and a suitable in vitro culture medium for hematopoietic stem cells;

[0012] 2) In the traditional method of hematopoietic stem cell transplantation, after the hematopoietic stem cells are collected from the body, the hematopoietic stem cells are usually placed in cryopreservation (freezing for autologous transplantation, but not for allogeneic transplantation, but both lack a rapid in vitro promotion). Activation process of anti-tumor activity) stored in liquid nitrogen at -196°C, and directly infused into recipients after thawing, so the activity and quantity of hematopoietic stem cells after preservation decreased;

[0013] 3) The hematopoietic stem cells after transplantation have limited ability to kill tumor cells, so the postoperative effect is average, and their ability to inhibit tumor growth needs to be further improved

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