Method for producing magnetosome by cultivating magnetotactic bacteria

A technology of magnetosomes and magnetobacteria, applied in the direction of bacteria, etc., can solve the problems of slow cell growth and achieve the effects of automatic feeding, simple operation, and cost reduction

Inactive Publication Date: 2011-08-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Japanese scholars have established a method of constant pH feeding and batch supplementation of iron sources when cultivating Magnetospira AMB-1, but the cell growth rate is slow, and only 0.58g / L dry cell weight can be harvested after 4 days of cultivation

Method used

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  • Method for producing magnetosome by cultivating magnetotactic bacteria
  • Method for producing magnetosome by cultivating magnetotactic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 The method of cultivating magnetobacteria to produce magnetosomes

[0032](1) In the culture medium containing 0.03g / L sodium lactate, 0.05g / L ammonium chloride and 0g / L ferric citrate, shake culture Magnetospira MSR-1, obtain seed culture solution, wherein said culture medium initial pH The value is 6.5;

[0033] (2) Add K-free in 42L automatic fermenter 2 HPO 4 The above culture medium was sterilized, continuously fed with sterile air (0.3L / min), and continuously stirred at 100rpm. When the temperature of the culture medium dropped below 40°C, add pre-sterilized K 2 HPO 4 The final concentration is 0.1g / L. When the temperature of the culture medium drops to 35°C, inoculate the seed solution activated in step (1) into the tank, and adjust and control the pH to 6.5 with hydrochloric acid;

[0034] (3) During the culture process, samples were taken every 2 hours to detect the cell density and lactic acid content and the iron content of the culture solution...

Embodiment 2

[0037] Example 2 The method of cultivating magnetobacteria to produce magnetosomes

[0038] (1) In the culture medium that contains 10.4g / L sodium lactate, 3.2g / L ammonium chloride and 2.08mg / L iron citrate, shake and cultivate Magnetospira MSR-1, obtain seed culture solution, wherein said culture medium initially The pH value is 7.5;

[0039] (2) Add K-free in 42L automatic fermenter 2 HPO 4 After sterilization, continue to pass sterile air (3L / min), and continuously stir at 100rpm. When the temperature of the medium drops below 40°C, add pre-sterilized K 2 HPO 4 The final concentration is 3g / L. When the temperature of the culture medium drops to 25°C, inoculate the seed solution activated in step (1) into the tank, and adjust and control the pH to 7.5 with lactic acid;

[0040] (3) During the culture process, samples were taken every 2 hours to detect the cell density and lactic acid content and the iron content of the culture solution, according to the empirical formul...

Embodiment 3

[0043] Example 3 The method of cultivating magnetobacteria to produce magnetosomes

[0044] (1) In the culture medium that contains 2.6g / L sodium lactate, 0.61g / L ammonium chloride and 13mg / L iron citrate, shake and cultivate Magnetospira MSR-1, obtain seed culture solution, wherein said culture medium initial pH Value is 7.0;

[0045] (2) Add K-free in 42L automatic fermenter 2 HPO 4 The above culture medium was sterilized, continuously fed with sterile air (0.3L / min), and continuously stirred at 100rpm. When the temperature of the culture medium dropped below 40°C, add pre-sterilized K 2 HPO 4 The final concentration is 0.5g / L. When the temperature of the culture medium drops to 28°C, inoculate the seed solution activated in step (1) into the tank, and adjust and control the pH to 7.0 with hydrochloric acid;

[0046] (3) Samples were taken every 2 hours during the culture process to detect cell density and lactic acid content ( figure 1 ) and determine the iron content...

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Abstract

The invention provides a method for producing magnetosome by cultivating magnetic bacteria, which takes air as an oxygen supplying way and maintains the continuous growth of the magnetic bacteria with a material feeding way, and when dissolved oxygen reaches or gets lower than the dissolved oxygen suitable for magnetosome production, the dissolved oxygen is controlled at a proper dissolved oxygenrange that is suitable for magnetosome production through agitating rotation speed, air flow speed and the dissolved oxygen in cascade. The magnetic bacteria cultivating method does not need air distribution and pure inert gases, but uses air aeration for oxygen supply, thus lowering initial dissolved oxygen from the saturation state of 100 percent to 0 percent, and the coordinated adjustment of agitating rotation speed and air flow speed can control the dissolved oxygen to be any value between the saturation states of 0 percent and 20 percent, thus lowering the production cost of magnetosomeand providing technical guarantee for researching the physiological and biochemical characteristics of the magnetic bacteria under different dissolved oxygen levels.

Description

technical field [0001] The invention relates to a bacterial culture method, in particular to a method for cultivating magnetobacteria to produce magnetosomes. Background technique [0002] Magnetotactic bacteria are a class of Gram-negative bacteria with magnetotropism, which can synthesize nano-magnetic particles that are arranged in chains and coated with lipid membranes—magnetosomes. Magnetosomes have the main properties and characteristics of excellent carriers, and have broad application prospects. However, it is limited to the laboratory level and has not yet been applied commercially. The main reason is that magnetotactic bacteria are difficult to cultivate artificially, and the synthesis conditions of magnetosomes are not easy to control. The reasons are: 1) magnetotactic bacteria have strict requirements on the nutrition in the environment, and can only use a few kinds of organic acids as carbon sources; 2) in nature Magnetotactic bacteria usually live in an envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 李颖孙建波姜伟田杰生王珍芳李季伦
Owner CHINA AGRI UNIV
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