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Gene therapy medicine for remedying erectile dysfunction and medicine box thereof

A technology for erectile dysfunction and gene therapy, applied in gene therapy, drug combination, pharmaceutical formulations, etc., can solve the problems of short time of expressing foreign genes, safety problems, side effects, and heavy immune response, and achieve long-lasting drug efficacy. , Clinical medication is convenient, the effect is faster

Inactive Publication Date: 2011-09-14
李学莹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although Dr. Mellman's research plan is perfect, there are still several thorny issues to be answered: 1. Using adenovirus (Ad) as a carrier, Ad has potential safety problems and side effects, which cannot be easily avoided; 2. The adenovirus The disadvantage is that the expression of foreign genes takes a short time and has strong immunogenicity, which can cause strong toxic and immune reactions in the body, high toxicity, severe immune reactions, drug resistance, and multiple injections; 3. The injection is directly on the penis Injection, users will have fear

Method used

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  • Gene therapy medicine for remedying erectile dysfunction and medicine box thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Gene therapy medicine of the present invention---the production technology of aqueous preparation is as follows:

[0044] (1) Cell seed expansion

[0045] Take the seed cells of the HEK293 cell bank required for a 50L fermenter, inoculate HEK293 into a special fermenter, preheat the fermenter medium to 37°C before inoculation, and use carbon dioxide to balance the pH of the culture medium. Automatic detection of various parameters, such as oxygen partial pressure, carbon dioxide partial pressure, pH value, temperature, glucose concentration, ammonia concentration, respiratory ratio, etc. It takes about 3-4 days for the cells to be cultured in the fermenter to the required culture density. The density of the cell growth is detected every day. The replacement and replenishment of the medium in the middle is based on the parameters displayed by the program-controlled instrument. The culture medium is a serum-free culture medium. .

[0046] (2) Plasmid transfection

[00...

Embodiment 2

[0057] Gene therapy medicine of the present invention---the production technology of instillation agent is as follows:

[0058] Except that step (6) is packaged as an infusion.

[0059] Other operations are the same as in Example 1.

Embodiment 3

[0061] Gene therapy medicine of the present invention---the production technology of injection is as follows:

[0062] Except that step (6) is packaged as an injection, 5% sorbitol is added as a protective solution.

[0063] Other operations are the same as in Example 1.

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Abstract

The invention provides a gene medicine for remedying the erectile dysfunction. The medicine is liquid, oil preparation, drop and injection of a potassium channel gene-adeno-associated virus vector recombinant and is prepared by separating and purifying by a three-plasmid contransfection method via the mass production cultivation of rAAV. The invention also provides a medicine box of the medicine.The medicine is simple and convenient for using, can effectively improve or remedy ED, has sustained effect, is safe without toxic or side effect and has great social and economic benefits.

Description

technical field [0001] The present invention belongs to the field of gene therapy, in particular, relates to a gene therapy drug for treating erectile dysfunction (Erectile Dysfunction, referred to as ED) and a kit thereof, more specifically, relates to a large potassium channel (maxi -K) A gene therapy drug of a gene-adeno-associated virus (AAV) vector recombinant and a kit thereof. Background technique [0002] A 6-month survey of more than 1,000 adult men conducted by Beijing People's Hospital in 2004 showed that 52.5% of Chinese men over the age of 40 suffer from impotence to varying degrees. A survey in the United States also shows that 40% of men aged 40-50 have had ED; and 70% of men aged 60-70. According to recent statistics, there are more than 30 million ED patients in the United States, and more than 100 million patients in the world. With the accelerated pace of life and the aging society, ED patients will increase year by year. In the past, male sexual dysfun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P15/10
Inventor 裘建英张云福
Owner 李学莹