Preparation and use method of microcystin-LR polyclonal antibody immunoaffinity column

A technology of microcystin and immunophile, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high price, low detection limit, interference detection results, etc.
CN101446575AActive Publication Date: 2009-06-03启东市三江建筑机械有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
启东市三江建筑机械有限公司
Publication Date
2009-06-03

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Abstract

Preparation and use methods of a microcystin-LR (MC-LR) polyclonal antibody immunoaffinity column (IAC) belong to the technical field of immunoaffinity chromatography and MC detection. The IAC is prepared by fixing MC-LR polyclonal antibody in the column, so that MC-LR in the sample solution can be adsorbed by the IAC and can be enriched and purified after elution, then the quantitative and qualitative analysis can be carried out by HPLC. As the pre-processing process, the IAC enrichment is superior to that of the prior solid-phase extraction (SPE) method. HPLC test shows that MC-LR peak of the MC-LR polyclonal antibody can be separated from peaks of other impurities by IAC so as not to influence the detection result. However, the SPE method is difficult to distinguish the MC-LR peak due to the presence of so many impurities. The MC-LR polyclonal antibody can be specifically combined with the MC-LR, so that IAC has excellent specificity to remove the majority of interferents in one step. The SPE has no specificity and cannot remove the interferents so as to influence the final detection result.
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Description

technical field

[0001] A method for preparing and using a microcystin-LR polyantibody immunoaffinity column belongs to the technical field of immunoaffinity chromatography and microcystin detection. Background technique

[0002] With the development of industry and agriculture and the acceleration of urbanization, industrial and domestic sewage enters the water environment, resulting in increased eutrophication of water bodies. The increase in the content of nitrogen and phosphorus in the water has caused the frequent occurrence of toxic cyanobacteria blooms. In recent years, my country's drinking water sources such as Taihu Lake, Chaohu Lake, Dianchi Lake and other lakes, some reservoirs and rivers have been attacked by cyanobacteria blooms. According to the "Report on the Protection and Development of the Yangtze River" released in April 2007, after the Three Gorges reservoir area was impounded to 135 meters in 2003, 12 first-class tributaries of the Yangtze River had "wa...

Claims

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