Abalone blood cell anticoagulation protecting agent and preparation method thereof

Abstract

The invention discloses an abalone blood cell anticoagulant protective agent and a preparation method thereof, and relates to an anticoagulant protective agent. The invention provides an abalone blood cell anticoagulant protective agent for maintaining the activity of isolated abalone blood cells within longer time and a preparation method thereof. The abalone blood cell anticoagulant protective agent comprises the following raw materials by mass percentage: 1.95 to 2.08 percent of glucose, 0.78 to 0.8 percent of sodium citrate (2H2O), 0.063 to 0.107 percent of citric acid, 0.37 to 0.57 percent of sodium chloride, 0.21 to 0.24 percent of hydroxyethyl piperazinyl ethyl thiosulfonic acid, and 100 percent of double distilled water, wherein the pH value is between 6.0 and 6.4. The abalone blood cell anticoagulant protective agent is obtained by dissolving the glucose, the sodium citrate (2H2O), the sodium chloride and the hydroxyethyl piperazinyl ethyl thiosulfonic acid into the double distilled water, stirring and dissolving the mixture, using the citric acid to adjust the pH value to be between 6.0 and 6.4, and performing high-temperature sterilization.

Description

technical field [0001] The invention relates to an anticoagulant protective agent, in particular to an anticoagulant protective agent for abalone blood cells and a preparation method thereof. Background technique [0002] Abalone is an important mariculture species in my country, rich in nutrients and high in economic value; however, it grows slowly and requires very clean aquaculture water. In recent years, the problem of abalone diseases has become more and more serious, and it has become a top priority to find effective methods to promote the healthy breeding of abalone. Similar to other shellfish, abalone's defense against invading pathogens is mainly achieved through the phagocytosis of foreign microorganisms by blood cells, so the study of abalone's blood cells is an important part of exploring its immune system. Abalone and other shellfish blood cells have a very remarkable characteristic. Without anticoagulant, once the blood cells are separated from the body, they ...

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Problems solved by technology

Although MAS anticoagulant can temporarily preserve the ex vivo blood cells of warty abalone (H.tuberculata) (1h), and is applied to the ex vivo preservation of European oyster blood cells (Xue, Q.G., Renault, T. and Chilmonczyk, S.: Flow cytometricassessment of haemocyte sub-populations in the European flat oyster, Ostrea edulis, haemolymph. Fish Shellfish Immunol 11 (2001) 557-567), but its anticoagulant effect on the blood cells of abalone and oyster has not been clearly reported

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