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Composition for regeneration of periodontal soft tissue and method for producing the same

A composition and soft tissue technology, applied in the direction of tissue regeneration, drug combination, medical preparations containing active ingredients, etc., can solve the problems of unfinished periodontal soft tissue low-invasive methods, unfinished, undiscovered, etc.

Inactive Publication Date: 2009-07-01
NAGOYA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the foregoing discussion, no less invasive methods of periodontal soft tissue regeneration have been studied to date, nor have such
Also, research on methods for effectively regenerating free gingiva such as interdental papillae has not been done, nor have such been found

Method used

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  • Composition for regeneration of periodontal soft tissue and method for producing the same
  • Composition for regeneration of periodontal soft tissue and method for producing the same
  • Composition for regeneration of periodontal soft tissue and method for producing the same

Examples

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preparation example Construction

[0091] The preparation method of this composition

[0092] There is no particular limitation on the preparation method of the present composition, for example, the method may only include simply mixing the constituent cells and matrix materials of the present composition with optional ingredients such as cell growth factors and the like. Buffered solutions, physiological saline and / or injectable solvents may be included as required. The number of cells in the present composition is preferably about 1.0 x 10 4 cells / mL~1.0×10 10 cells / mL.

[0093]A variety of matrix materials have been described that can be used as a cell buffer matrix, and are preferably used selected from (a) hyaluronic acid and its derivatives, (b) collagen and its derivatives, (c) fibrin and fibrin Progen and (d) one or more of plasma and platelets (usually PRP). The matrix material solution used in the present composition can have hyaluronic acid at a concentration of, for example, 3 mg / mL-15 mg / mL, pr...

Embodiment 1

[0120] Example 1: Preparation of human mesenchymal stem cells

[0121] Before surgery, harvesting was performed from the patient's ilium by an ordinary method. Inoculate the collected bone marrow aspirate with appropriate culture media into tissue culture dishes. Suspended cells were removed, and cells were cultured and propagated with medium changed at regular intervals.

Embodiment 2

[0122] Example 2: Preparation of human gingival fibroblasts

[0123] Before the procedure, the samples were collected from the patient's gums. Inoculate culture dishes by transplantation and inoculate at 37 °C in 5% CO 2 cultured in an incubator. Cell blocks were cultured, propagated and prepared by general methods (the same as those used for undifferentiated mesenchymal stem cells).

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Abstract

It is intended to provide a composition for regeneration of periodontal soft tissue less invasively and a method for producing the composition. The composition for regeneration of periodontal soft tissue is prepared so as to contain a cell selected from an undifferentiated / stem cell or a blast cell having an ability to form gingiva, a matrix material and blood platelet plasma. With the composition, free gingiva as well as attached gingiva can be regenerated less invasively with an effective and good aesthetics.

Description

technical field [0001] The present invention relates to a composition for periodontal soft tissue regeneration and a preparation method of the composition. Background technique [0002] In recent years, various oral surgical treatments for periodontal disease have been realized. In periodontal disease, a portion of the periodontal tissue is receded or lost, either in its own infected area or as a result of surgical treatment thereof. However, in order to ensure functionality and maintain aesthetics after treatment, there is an increasing demand for the repair and regeneration of such periodontal tissues. [0003] Periodontal tissue consists of hard tissue (such as the alveolar bone that supports the teeth) and soft tissue (such as the gums). Such as figure 1 As shown, soft periodontal tissue includes attached gingiva attached to the alveolar bone and free gingiva not attached to this hard tissue. [0004] Regarding the repair and regeneration of periodontal tissue, for e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00A61K31/728A61K35/12A61K35/28A61K35/32A61L27/20A61L27/36A61L27/38A61L27/40A61L27/44
CPCA61L27/3865A61L2430/12A61L27/3804A61L27/3834A61P19/04
Inventor 山田阳一上田实冈部一登高后友之
Owner NAGOYA UNIVERSITY