Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant diseases inoculation tool and application thereof in inoculation by needle punching method

A plant disease and inoculation needle technology, which is applied in the field of inoculation tools for plant pathogenic bacteria, can solve the problems of low inoculation efficiency, insufficient stability of inoculation effect, and inconvenient use, and achieve high inoculation efficiency, stable inoculation effect, and favorable processing effects

Inactive Publication Date: 2010-09-01
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to: in view of the practical problems of low inoculation efficiency, inoculation effect not stable enough, inconvenient to use, and affecting accurate and appropriate evaluation of the resistance of varieties and pathogenic bacteria in the use of current acupuncture inoculation tools, to provide A plant disease inoculation tool and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant diseases inoculation tool and application thereof in inoculation by needle punching method
  • Plant diseases inoculation tool and application thereof in inoculation by needle punching method
  • Plant diseases inoculation tool and application thereof in inoculation by needle punching method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Use medical pliers to make plant disease inoculation tools:

[0026] Production of the A part:

[0027] The inoculation pad 2 is a rectangular rubber plate of 8×6×4mm (length×width×thickness), and four needle-punched holes 3 are made with a punching machine. The needle-punched holes 3 are circular holes with a diameter of 1mm, and they are distributed in a (length×width) rectangle corners, the center of the hole is 1.5mm from the edge of the long side, and 1mm from the edge of the short side. After the production is completed, the long symmetrical central axis of the piece A coincides with a jaw and is fixed at the front end of the jaw. Fixing can be pasted with 502 glue. If it is produced industrially, the piece A can be directly fixed on the jaws of the medical forceps.

[0028] Production of Part B:

[0029] The backing plate 4 is a rectangle of 8×6×0.5mm (length×width×thickness) cut from a copper or aluminum sheet, the inoculation plate 5 is a rectangular rubber...

Embodiment 2

[0032] Phytopathogenic bacteria:

[0033] Bacterial streak bacterium JSb1-1 was transplanted on Clutch Ben's medium, cultured for 48 hours, washed with sterile water, and the pathogenic bacteria were prepared into a bacterial suspension of 300 million cells / ml for inoculation.

[0034] Inoculation method:

[0035] Acupuncture

[0036] use tools:

[0037] Tools made for conventional inoculation tools and examples, respectively

[0038] Test species and inoculation timing:

[0039] The rice variety King Kong 30 is in the peak tillering stage

[0040] Survey time:

[0041] The results of the survey 21 days after inoculation, the results of the survey are shown in Table 1

[0042] Table 1: The incidence of pathogenic bacteria JSB1-1 after inoculation with two inoculation tools

[0043]

[0044] Conclusion: The incidence of the inoculation of the present invention is comparable to that of traditional inoculation tools, and the disease after inoculation of the present inve...

Embodiment 3

[0046] The difference between this embodiment and embodiment 2 is only: the phytopathogenic bacteria, the species tested and the timing of inoculation are different. in:

[0047] Phytopathogenic bacteria:

[0048] Bacterial streak bacterium GD414 was transplanted on the slanted Ben's medium, cultured for 48 hours, washed with sterile water, and the pathogenic bacteria were prepared into a bacterial suspension of 300 million cells / ml for inoculation.

[0049] Test species and inoculation timing:

[0050] Zhengxuan 2, Jinke 3, Zhengdao 040285, D0J01 (H040744), D0J08 (Lianjing 05045), Chijing 12, Chijingsheng 01, the growth period of the varieties is at the booting stage.

[0051] The survey results are shown in Table 2

[0052] Table 2: The incidence of pathogenic bacteria GD414 inoculated with two inoculation tools on different varieties

[0053]

[0054] Conclusion: The incidence of the same pathogenic bacteria on different varieties is stable and repeatable, whi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a plant disease inoculation tool, which comprises a pincers body and an inoculation strip provided with an A sheet and a B sheet which correspond to each other, wherein the A sheet is an inoculation pad, and pinpricks are arranged in the inoculation pad; the B sheet comprises a padding plate and an inoculation plate, and the inoculation plate is connected with jaws through the padding plate; inoculation needles are arranged on the inoculation plate, and a bacteria liquid fixing piece is covered on the inoculation plate. The application of the plant disease inoculationtool in a pin-prick inoculation is as follows: plant pathogenic bacteria are prepared into 300 million cells / ml of bacteria suspension after being cultivated; the bacteria liquid fixing piece is covered on the inoculation plate after being immersed in the bacteria suspension, and the needlepoints of the inoculation needles penetrate through the bacteria liquid fixing piece to be exposed outside; leaves are placed between the A sheet inoculation pad of the inoculation strip and the B sheet, the jaws are closed to enable the inoculation needles to penetrate through the leaves into the pinpricksof the A sheet inoculation pad, and the plant pathogenic bacteria in the bacteria liquid fixing piece are inoculated into the leaves by slightly pressing.

Description

technical field [0001] The invention relates to a tool for inoculating plant pathogenic bacteria and its application, in particular to a tool for inoculating plant pathogenic bacteria through acupuncture and its application. Background technique [0002] Inoculation of plant diseases is an important method for the virulence of plant pathogens, the identification of pathogens and their host disease resistance. The determination of the virulence of pathogenic bacteria and the identification of pathogenic bacteria must be done through the inoculation of pathogenic bacteria. Among them, efficient and simple inoculation tools are very important, and they are of great significance to the research on the resistance of pathogenic bacteria and hosts. [0003] Acupuncture inoculation is an important inoculation method for plant pathogenic bacteria. Acupuncture inoculation is the main method for bacterial inoculation of plant pathogenic bacteria, but there is a lack of efficient and c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G7/06
Inventor 刘永锋陈志谊刘邮洲罗楚平聂亚峰
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com